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Assay Detail

CHEMBL5336804

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of mouse GAT3 transfected in HEK293 cells assessed as reduction in [3H]-GABA uptake preincubated for 25 mins followed by [3H]-GABA addition and measured after 4 mins by liquid scintillation counting analysis
35
Total Activities
31
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Cell Type HEK293
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Discovery of novel multifunctional ligands targeting GABA transporters, butyrylcholinesterase, β-secretase, and amyloid β aggregation as potential treatment of Alzheimer's disease.
Zaręba P, Łątka K, Mazur G, Gryzło B, Pasieka A, Godyń J, Panek D, Skrzypczak-Wiercioch A, Höfner GC, Latacz G, Maj M, Espargaró A, Sabaté R, Jóźwiak K, Wanner KT, Sałat K, Malawska B, Kulig K, Bajda M.
Eur J Med Chem (2023) 261 : 115832 -115832
PubMed 37837674 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 35 4.96 5.39

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5419680 2 5.39
CHEMBL5423337 1 5.21
CHEMBL5423316 1 5.20
CHEMBL5404846 1 5.18
CHEMBL5409309 1 5.11
CHEMBL5421481 1 5.11
CHEMBL5412629 1 5.10
CHEMBL5405236 1 5.10
CHEMBL5411905 1 5.08
CHEMBL5419107 1 5.07
CHEMBL5412199 1 5.07
CHEMBL5403788 2 5.05
CHEMBL5409538 1 5.05
CHEMBL5411369 1 5.00
CHEMBL5414446 1 4.99
CHEMBL5413754 1 4.97
CHEMBL5407267 1 4.96
CHEMBL5398245 1 4.93
CHEMBL5416293 1 4.89
CHEMBL5435638 1 4.88
CHEMBL5424877 2 4.87
CHEMBL5394533 2 4.86
CHEMBL5416743 1 4.83
CHEMBL5440565 1 4.82
CHEMBL5423098 1 4.79
CHEMBL5423441 1 4.77
CHEMBL5425025 1 4.77
CHEMBL5438498 1 4.76
CHEMBL5411893 1 4.74
CHEMBL5397274 1 4.64

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5419680 IC50 = 4073.8 nM 5.39
CHEMBL5419680 IC50 = 4100.0 nM 5.39
CHEMBL5423337 IC50 = 6165.95 nM 5.21
CHEMBL5423316 IC50 = 6309.57 nM 5.20
CHEMBL5404846 IC50 = 6606.93 nM 5.18
CHEMBL5421481 IC50 = 7762.47 nM 5.11
CHEMBL5409309 IC50 = 7762.47 nM 5.11
CHEMBL5405236 IC50 = 7943.28 nM 5.10
CHEMBL5412629 IC50 = 7943.28 nM 5.10
CHEMBL5411905 IC50 = 8317.64 nM 5.08
CHEMBL5419107 IC50 = 8511.38 nM 5.07
CHEMBL5412199 IC50 = 8511.38 nM 5.07
CHEMBL5403788 IC50 = 8912.51 nM 5.05
CHEMBL5409538 IC50 = 8912.51 nM 5.05
CHEMBL5411369 IC50 = 10000.0 nM 5.00
CHEMBL5414446 IC50 = 10232.93 nM 4.99
CHEMBL5413754 IC50 = 10715.19 nM 4.97
CHEMBL5407267 IC50 = 10964.78 nM 4.96
CHEMBL5398245 IC50 = 11748.98 nM 4.93
CHEMBL5416293 IC50 = 12882.5 nM 4.89
CHEMBL5435638 IC50 = 13182.57 nM 4.88
CHEMBL5403788 IC50 = 13489.63 nM 4.87
CHEMBL5424877 IC50 = 13489.63 nM 4.87
CHEMBL5394533 IC50 = 13803.84 nM 4.86
CHEMBL5416743 IC50 = 14791.08 nM 4.83
CHEMBL5440565 IC50 = 15135.61 nM 4.82
CHEMBL5394533 IC50 = 15135.61 nM 4.82
CHEMBL5423098 IC50 = 16218.1 nM 4.79
CHEMBL5423441 IC50 = 16982.44 nM 4.77
CHEMBL5425025 IC50 = 16982.44 nM 4.77
CHEMBL5438498 IC50 = 17378.01 nM 4.76
CHEMBL5411893 IC50 = 18197.01 nM 4.74
CHEMBL5397274 IC50 = 22908.68 nM 4.64
CHEMBL5424877 IC50 = 25118.86 nM 4.60
CHEMBL5397616 IC50 = 26302.68 nM 4.58