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Assay Detail

CHEMBL5337141

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Binding
Inhibition of N-terminal His-tagged recombinant human TET2 catalytic domain LCIdel mutant (D1129 to G1936 residues with Y1481 to N1843 replaced by a 3 x GGGGS linker) expressed in Escherichia coli BL21 (DE3) using 5-methylcytosine as substrate preincubated for 10 mins followed by DNA-cofactor addition and measured after 10 mins by Alphascreen assay
18
Total Activities
16
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Focused Screening Identifies Different Sensitivities of Human TET Oxygenases to the Oncometabolite 2-Hydroxyglutarate.
Belle R, Saraç H, Salah E, Bhushan B, Szykowska A, Roper G, Tumber A, Kriaucionis S, Burgess-Brown N, Schofield CJ, Brown T, Kawamura A.
J Med Chem (2024) 67 : 4525 -4540
PubMed 38294854 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 15 5.31 6.09
pIC50 3 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1398529 1 6.09
CHEMBL483254 PANOBINOSTAT 4.0 1 5.68
CHEMBL1230640 1 5.59
CHEMBL1982368 1 5.45
CHEMBL4475458 1 5.33
CHEMBL1615211 2 5.22
CHEMBL3646221 VADADUSTAT 4.0 1 5.21
CHEMBL1614745 2 5.14
CHEMBL90852 N-OXALYLGLYCINE 1 5.12
CHEMBL316034 1 5.01
CHEMBL3646117 1 4.80
CHEMBL576 SUCCINIC ACID 3.0 1 -
CHEMBL503160 FUMARIC ACID 3.0 1 -
CHEMBL1234242 1 -
CHEMBL597779 1 -
CHEMBL4877741 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1398529 IC50 = 812.83 nM 6.09
CHEMBL483254 PANOBINOSTAT IC50 = 2089.3 nM 5.68
CHEMBL1230640 IC50 = 2570.4 nM 5.59
CHEMBL1982368 IC50 = 3548.13 nM 5.45
CHEMBL4475458 IC50 = 4677.35 nM 5.33
CHEMBL1615211 IC50 = 6000.0 nM 5.22
CHEMBL3646221 VADADUSTAT IC50 = 6165.95 nM 5.21
CHEMBL1615211 IC50 = 6165.95 nM 5.21
CHEMBL1614745 IC50 = 7244.36 nM 5.14
CHEMBL1614745 IC50 = 7200.0 nM 5.14
CHEMBL90852 N-OXALYLGLYCINE IC50 = 7585.78 nM 5.12
CHEMBL316034 IC50 = 9772.37 nM 5.01
CHEMBL3646117 IC50 = 15848.93 nM 4.80
CHEMBL576 SUCCINIC ACID IC50 = 104712.85 nM -
CHEMBL503160 FUMARIC ACID IC50 = 158489.32 nM -
CHEMBL1234242 pIC50 - -
CHEMBL597779 pIC50 - -
CHEMBL4877741 pIC50 - -