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Assay Detail

CHEMBL5337969

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of C-terminal NanoLuc-tagged MPGLWKS peptide from N-terminal HaloTag-tagged GID4 (unknown origin) expressed in HEK293T cells incubated for 4 hrs by fluorescence polarization based NanoBRET assay
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293T
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Glucose-induced degradation protein 4 homolog (CHEMBL5169174)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Chemical tools for the Gid4 subunit of the human E3 ligase C-terminal to LisH (CTLH) degradation complex.
Yazdi AK, Perveen S, Dong C, Song X, Dong A, Szewczyk MM, Calabrese MF, Casimiro-Garcia A, Chakrapani S, Dowling MS, Ficici E, Lee J, Montgomery JI, O'Connell TN, Skrzypek GJ, Tran TP, Troutman MD, Wang F, Young JA, Min J, Barsyte-Lovejoy D, Brown PJ, Santhakumar V, Arrowsmith CH, Vedadi M, Owen DR.
RSC Med Chem (2024) 15 : 1066 -1071
PubMed 38516600 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 5.60 5.60

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5411984 1 5.60

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5411984 IC50 = 2500.0 nM 5.60