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Assay Detail

CHEMBL5342456

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to autoinhibited CSF1R (538 to 939) (unknown origin) expressed in Escherichia coli BL21 strain assessed as dissociation constant incubated for 1 hr under shaking condition by qPCR method
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Macrophage colony-stimulating factor 1 receptor (CHEMBL1844)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and Development of Highly Selective Pyrrolo[2,3-<i>d</i>]pyrimidine CSF1R Inhibitors Targeting the Autoinhibited Form.
Aarhus TI, Bjørnstad F, Wolowczyk C, Larsen KU, Rognstad L, Leithaug T, Unger A, Habenberger P, Wolf A, Bjørkøy G, Pridans C, Eickhoff J, Klebl B, Hoff BH, Sundby E.
J Med Chem (2023) 66 : 6959 -6980
PubMed 37191268 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 10 7.71 8.64

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5439126 1 8.64
CHEMBL5436096 1 8.21
CHEMBL5436321 1 8.17
CHEMBL5418087 1 8.14
CHEMBL5397490 1 8.10
CHEMBL5406924 1 7.58
CHEMBL5440766 1 7.42
CHEMBL5405639 1 7.28
CHEMBL5417945 1 7.14
CHEMBL3813873 PEXIDARTINIB 4.0 1 6.44

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5439126 Kd = 2.3 nM 8.64
CHEMBL5436096 Kd = 6.1 nM 8.21
CHEMBL5436321 Kd = 6.8 nM 8.17
CHEMBL5418087 Kd = 7.2 nM 8.14
CHEMBL5397490 Kd = 7.9 nM 8.10
CHEMBL5406924 Kd = 26.0 nM 7.58
CHEMBL5440766 Kd = 38.0 nM 7.42
CHEMBL5405639 Kd = 52.0 nM 7.28
CHEMBL5417945 Kd = 72.0 nM 7.14
CHEMBL3813873 PEXIDARTINIB Kd = 360.0 nM 6.44