Assay Detail
Binding
CHEMBL5342659
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of BTK in HEK293 cells using NanoGlo substrate incubated for 2 hrs by NanoBRET assay
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | HEK293 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Discovery of Novel Bruton's Tyrosine Kinase PROTACs with Enhanced Selectivity and Cellular Efficacy.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 5 | 6.73 | 7.92 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4065122 | FENEBRUTINIB | 3.0 | 1 | 7.92 |
| CHEMBL5408743 | — | — | 1 | 7.01 |
| CHEMBL5403472 | — | — | 1 | 6.50 |
| CHEMBL5437508 | — | — | 1 | 5.50 |
| CHEMBL43452 | POMALIDOMIDE | 4.0 | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4065122 | FENEBRUTINIB | IC50 | = | 12.1 | nM | 7.92 |
| CHEMBL5408743 | — | IC50 | = | 97.0 | nM | 7.01 |
| CHEMBL5403472 | — | IC50 | = | 320.0 | nM | 6.50 |
| CHEMBL5437508 | — | IC50 | = | 3200.0 | nM | 5.50 |
| CHEMBL43452 | POMALIDOMIDE | IC50 | - | — | - |