Assay Detail
Binding
CHEMBL5347341
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of human recombinant full length METTL3/human N-terminal FLAG-tagged METTL14 expressed in SF9 cells assessed as methyltransferase residual activity using ssRNA 5'-rUrArC rArCrU rCrGrA rUrCrU rGrGrA rCrUrA rArArG rCrUrG rCrUrC -3' as substrate incubated for 20 mins followed by H-SAM addition measured after 2 hrs by radio isotope reaction system analysis
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | Sf9 |
| Confidence | 7 — Homologous single protein target |
| Curated By | Autocuration |
Publication
Rational design of novel nucleoside analogues reveals potent antiviral agents for EV71.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 3 | 4.23 | 4.23 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5426548 | — | — | 1 | 4.23 |
| CHEMBL5431300 | — | — | 1 | - |
| CHEMBL5409037 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5426548 | — | IC50 | = | 58800.0 | nM | 4.23 |
| CHEMBL5431300 | — | IC50 | = | 184000.0 | nM | - |
| CHEMBL5409037 | — | IC50 | = | 129000.0 | nM | - |