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Assay Detail

CHEMBL5347938

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human ATX using LPC as substrate preincubated with compound for 15 mins followed by substrate addition measured after 30 mins by Amplex Red reagent based fluorescence analysis
33
Total Activities
33
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Autotaxin (CHEMBL3691)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

"Hit" to lead optimization and chemoinformatic studies for a new series of Autotaxin inhibitors.
Stylianaki EA, Magkrioti C, Ladopoulou EM, Papavasileiou KD, Lagarias P, Melagraki G, Samiotaki M, Panayotou G, Dedos SG, Afantitis A, Aidinis V, Matralis AN.
Eur J Med Chem (2023) 249 : 115130 -115130
PubMed 36702053 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 33 5.06 6.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3828074 ZIRITAXESTAT 3.0 1 6.70
CHEMBL5434573 1 6.05
CHEMBL5408703 1 5.70
CHEMBL5402883 1 5.34
CHEMBL5415947 1 5.28
CHEMBL5408051 1 5.18
CHEMBL5427479 1 5.17
CHEMBL5422291 1 5.12
CHEMBL5428151 1 4.95
CHEMBL5439111 1 4.92
CHEMBL5440421 1 4.80
CHEMBL5396556 1 4.67
CHEMBL5418211 1 4.61
CHEMBL5427845 1 4.60
CHEMBL5408393 1 4.43
CHEMBL5425515 1 4.38
CHEMBL5413441 1 4.16
CHEMBL5401345 1 -
CHEMBL5418016 1 -
CHEMBL5410920 1 -
CHEMBL5422275 1 -
CHEMBL5405766 1 -
CHEMBL5397676 1 -
CHEMBL5438381 1 -
CHEMBL5413900 1 -
CHEMBL5432113 1 -
CHEMBL5423018 1 -
CHEMBL5412570 1 -
CHEMBL5403453 1 -
CHEMBL5404774 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3828074 ZIRITAXESTAT IC50 = 200.0 nM 6.70
CHEMBL5434573 IC50 = 900.0 nM 6.05
CHEMBL5408703 IC50 = 2000.0 nM 5.70
CHEMBL5402883 IC50 = 4600.0 nM 5.34
CHEMBL5415947 IC50 = 5300.0 nM 5.28
CHEMBL5408051 IC50 = 6600.0 nM 5.18
CHEMBL5427479 IC50 = 6700.0 nM 5.17
CHEMBL5422291 IC50 = 7600.0 nM 5.12
CHEMBL5428151 IC50 = 11200.0 nM 4.95
CHEMBL5439111 IC50 = 12100.0 nM 4.92
CHEMBL5440421 IC50 = 16000.0 nM 4.80
CHEMBL5396556 IC50 = 21200.0 nM 4.67
CHEMBL5418211 IC50 = 24400.0 nM 4.61
CHEMBL5427845 IC50 = 25000.0 nM 4.60
CHEMBL5408393 IC50 = 37300.0 nM 4.43
CHEMBL5425515 IC50 = 41800.0 nM 4.38
CHEMBL5413441 IC50 = 70000.0 nM 4.16
CHEMBL5401345 IC50 - -
CHEMBL5418016 IC50 - -
CHEMBL5410920 IC50 - -
CHEMBL5422275 IC50 > 100000.0 nM -
CHEMBL5405766 IC50 > 100000.0 nM -
CHEMBL5397676 IC50 > 100000.0 nM -
CHEMBL5438381 IC50 - -
CHEMBL5413900 IC50 - -
CHEMBL5432113 IC50 - -
CHEMBL5423018 IC50 - -
CHEMBL5412570 IC50 > 50000.0 nM -
CHEMBL5403453 IC50 - -
CHEMBL5404774 IC50 - -
CHEMBL5432871 IC50 - -
CHEMBL5437000 IC50 - -
CHEMBL5414417 IC50 > 100000.0 nM -