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Assay Detail

CHEMBL5349003

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of BRD4 BD2 (unknown origin) by TR-FRET assay
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Bromodomain-containing protein 4 (CHEMBL1163125)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

From PROTAC to inhibitor: Structure-guided discovery of potent and orally bioavailable BET inhibitors.
Koravovic M, Mayasundari A, Tasic G, Keramatnia F, Stachowski TR, Cui H, Chai SC, Jonchere B, Yang L, Li Y, Fu X, Hiltenbrand R, Paul L, Mishra V, Klco JM, Roussel MF, Pomerantz WC, Fischer M, Rankovic Z, Savic V.
Eur J Med Chem (2023) 251 : 115246 -115246
PubMed 36898329 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 8.86 10.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5424952 1 10.00
CHEMBL5440537 1 9.70
CHEMBL5432745 1 9.70
CHEMBL5401253 1 9.52
CHEMBL5440963 1 9.52
CHEMBL5422881 1 9.40
CHEMBL5403848 1 9.22
CHEMBL5436475 1 9.15
CHEMBL5422163 1 9.00
CHEMBL5439607 1 9.00
CHEMBL5400310 1 8.52
CHEMBL5399489 1 8.22
CHEMBL3581647 BIRABRESIB 2.0 1 8.05
CHEMBL1957266 1 7.89
CHEMBL5420752 1 7.58
CHEMBL5398000 1 7.22

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5424952 IC50 = 0.1 nM 10.00
CHEMBL5440537 IC50 = 0.2 nM 9.70
CHEMBL5432745 IC50 = 0.2 nM 9.70
CHEMBL5401253 IC50 = 0.3 nM 9.52
CHEMBL5440963 IC50 = 0.3 nM 9.52
CHEMBL5422881 IC50 = 0.4 nM 9.40
CHEMBL5403848 IC50 = 0.6 nM 9.22
CHEMBL5436475 IC50 = 0.7 nM 9.15
CHEMBL5422163 IC50 = 1.0 nM 9.00
CHEMBL5439607 IC50 = 1.0 nM 9.00
CHEMBL5400310 IC50 = 3.0 nM 8.52
CHEMBL5399489 IC50 = 6.0 nM 8.22
CHEMBL3581647 BIRABRESIB IC50 = 9.0 nM 8.05
CHEMBL1957266 IC50 = 13.0 nM 7.89
CHEMBL5420752 IC50 = 26.0 nM 7.58
CHEMBL5398000 IC50 = 60.0 nM 7.22