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Assay Detail

CHEMBL5350656

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]DTG from sigma 2 receptor in Sprague-Dawley rat liver assessed as inhibition constant by liquid scintillation counter analysis
22
Total Activities
22
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Liver
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sigma intracellular receptor 2 (CHEMBL4105864)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Dual Piperidine-Based Histamine H<sub>3</sub> and Sigma-1 Receptor Ligands in the Treatment of Nociceptive and Neuropathic Pain.
Szczepańska K, Karcz T, Dichiara M, Mogilski S, Kalinowska-Tłuścik J, Pilarski B, Leniak A, Pietruś W, Podlewska S, Popiołek-Barczyk K, Humphrys LJ, Ruiz-Cantero MC, Reiner-Link D, Leitzbach L, Łażewska D, Pockes S, Górka M, Zmysłowski A, Calmels T, Cobos EJ, Marrazzo A, Stark H, Bojarski AJ, Amata E, Kieć-Kononowicz K.
J Med Chem (2023) 66 : 9658 -9683
PubMed 37418295 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 22 7.42 8.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5436799 1 8.40
CHEMBL462605 PITOLISANT 4.0 1 8.19
CHEMBL5427817 1 8.00
CHEMBL5433976 1 8.00
CHEMBL5440901 1 7.80
CHEMBL5432307 1 7.77
CHEMBL4597895 1 7.72
CHEMBL4776141 1 7.65
CHEMBL5421122 1 7.60
CHEMBL4159046 1 7.57
CHEMBL5404813 1 7.54
CHEMBL5413280 1 7.48
CHEMBL4598396 1 7.34
CHEMBL5411175 1 7.33
CHEMBL5419356 1 7.33
CHEMBL5398497 1 7.19
CHEMBL1823340 1 7.17
CHEMBL4743583 1 7.12
CHEMBL4783744 1 7.00
CHEMBL5431002 1 6.99
CHEMBL5413694 1 6.94
CHEMBL2170062 1 5.03

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5436799 Ki = 4.0 nM 8.40
CHEMBL462605 PITOLISANT Ki = 6.5 nM 8.19
CHEMBL5427817 Ki = 9.9 nM 8.00
CHEMBL5433976 Ki = 10.0 nM 8.00
CHEMBL5440901 Ki = 16.0 nM 7.80
CHEMBL5432307 Ki = 17.0 nM 7.77
CHEMBL4597895 Ki = 19.0 nM 7.72
CHEMBL4776141 Ki = 22.4 nM 7.65
CHEMBL5421122 Ki = 25.0 nM 7.60
CHEMBL4159046 Ki = 27.0 nM 7.57
CHEMBL5404813 Ki = 29.0 nM 7.54
CHEMBL5413280 Ki = 33.0 nM 7.48
CHEMBL4598396 Ki = 46.0 nM 7.34
CHEMBL5411175 Ki = 47.0 nM 7.33
CHEMBL5419356 Ki = 47.0 nM 7.33
CHEMBL5398497 Ki = 65.0 nM 7.19
CHEMBL1823340 Ki = 68.0 nM 7.17
CHEMBL4743583 Ki = 75.2 nM 7.12
CHEMBL4783744 Ki = 101.0 nM 7.00
CHEMBL5431002 Ki = 103.0 nM 6.99
CHEMBL5413694 Ki = 116.0 nM 6.94
CHEMBL2170062 Ki = 9300.0 nM 5.03