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Assay Detail

CHEMBL5350928

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Inhibition of colony formation in human HCT-116 cells preincubated for 1 hr followed by exposure to 2 Gy gamma irradiation and measured after 1 week by crystal violet staining based chemiluminescence assay
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HCT-116
Confidence 1 — Organism
Curated By Autocuration

Target

HCT-116 (CHEMBL394)
Type CELL-LINE
Organism Homo sapiens

Publication

Discovery and Evaluation of 3-Quinoxalin Urea Derivatives as Potent, Selective, and Orally Available ATM Inhibitors Combined with Chemotherapy for the Treatment of Cancer via Goal-Oriented Molecule Generation and Virtual Screening.
Deng D, Yang Y, Zou Y, Liu K, Zhang C, Tang M, Yang T, Chen Y, Yuan X, Guo Y, Zhang S, Si W, Peng B, Xu Q, He W, Xu D, Xiang M, Chen L.
J Med Chem (2023) 66 : 9495 -9518
PubMed 37438997 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 17 8.27 9.34

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3960662 1 9.34
CHEMBL5411336 1 9.27
CHEMBL5402194 1 8.81
CHEMBL5419324 1 8.77
CHEMBL5425392 1 8.75
CHEMBL5434323 1 8.36
CHEMBL5429982 1 8.27
CHEMBL5423087 1 8.16
CHEMBL5419474 1 8.15
CHEMBL5438394 1 8.12
CHEMBL5415162 1 8.05
CHEMBL5407799 1 7.91
CHEMBL5416391 1 7.81
CHEMBL5438262 1 7.81
CHEMBL4218734 1 7.70
CHEMBL5398961 1 7.66
CHEMBL5406188 1 7.58

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3960662 IC50 = 0.46 nM 9.34
CHEMBL5411336 IC50 = 0.54 nM 9.27
CHEMBL5402194 IC50 = 1.56 nM 8.81
CHEMBL5419324 IC50 = 1.69 nM 8.77
CHEMBL5425392 IC50 = 1.76 nM 8.75
CHEMBL5434323 IC50 = 4.35 nM 8.36
CHEMBL5429982 IC50 = 5.41 nM 8.27
CHEMBL5423087 IC50 = 6.97 nM 8.16
CHEMBL5419474 IC50 = 7.13 nM 8.15
CHEMBL5438394 IC50 = 7.65 nM 8.12
CHEMBL5415162 IC50 = 8.94 nM 8.05
CHEMBL5407799 IC50 = 12.22 nM 7.91
CHEMBL5416391 IC50 = 15.35 nM 7.81
CHEMBL5438262 IC50 = 15.42 nM 7.81
CHEMBL4218734 IC50 = 20.02 nM 7.70
CHEMBL5398961 IC50 = 21.91 nM 7.66
CHEMBL5406188 IC50 = 26.47 nM 7.58