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Assay Detail

CHEMBL5351762

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human HDAC4 expressed in baculovirus expression system in Sf9 cells using Boc-Lys(trifluoroacetyl)-AMC fluorogenic peptide as substrate and measured after 2 hrs by fluorescence based analysis
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histone deacetylase 4 (CHEMBL3524)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of HDAC6, HDAC8, and 6/8 Inhibitors and Development of Cell-Based Drug Screening Models for the Treatment of TGF-β-Induced Idiopathic Pulmonary Fibrosis.
Yu WC, Yeh TY, Ye CH, Chong PCT, Ho YH, So DK, Yap KY, Peng GR, Shao CH, Jagtap AD, Chern JW, Lin CS, Lin SP, Lin SL, Yu SH, Yu CW.
J Med Chem (2023) 66 : 10528 -10557
PubMed 37463500 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 5.41 6.29

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5438457 1 6.29
CHEMBL2425955 1 4.52

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5438457 IC50 = 516.0 nM 6.29
CHEMBL2425955 IC50 = 30000.0 nM 4.52