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Assay Detail

CHEMBL5351970

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of interaction of PD-1 (unknown origin) expressed in Jurkat T cells carrying luciferase reporter under the control of NFAT promoter/PD-L1 (unknown origin) expressed in CHO cells co-expressing TCR ligand and T-cell surrogate pretreated with CHO cells for 0.5 hrs followed by Jurkat T cell addition and measured after 5 hrs by luminescence based analysis
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Jurkat
Confidence 7 — Homologous single protein target
Curated By Autocuration

Publication

Discovery of Novel d-(+)-Biotin-Conjugated Resorcinol Dibenzyl Ether-Based PD-L1 Inhibitors for Targeted Cancer Immunotherapy.
Ding Z, Wang S, Shi Y, Fei X, Cheng B, Lu Y, Chen J.
J Med Chem (2023) 66 : 10364 -10380
PubMed 37480153 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 2 5.69 6.04

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5403275 1 6.04
CHEMBL4802680 1 5.33

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5403275 EC50 = 920.0 nM 6.04
CHEMBL4802680 EC50 = 4650.0 nM 5.33