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Assay Detail

CHEMBL5354106

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of MB-COMT in rat liver homogenates using adrenaline as substrate and SAM as cofactor preincubated for 20 mins followed by substrate addition and measured after 5 mins
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Liver
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Catechol O-methyltransferase (CHEMBL2372)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Boosting caffeic acid performance as antioxidant and monoamine oxidase B/catechol-O-methyltransferase inhibitor.
Chavarria D, Benfeito S, Soares P, Lima C, Garrido J, Serrão P, Soares-da-Silva P, Remião F, Oliveira PJ, Borges F.
Eur J Med Chem (2022) 243 : 114740 -114740
PubMed 36116233 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 5.70 7.77

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1324 TOLCAPONE 4.0 1 7.77
CHEMBL5410682 1 5.57
CHEMBL5436270 1 5.44
CHEMBL5421285 1 5.43
CHEMBL2018554 1 5.42
CHEMBL5400279 1 5.40
CHEMBL5418340 1 5.37
CHEMBL5422642 1 5.21

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1324 TOLCAPONE IC50 = 17.0 nM 7.77
CHEMBL5410682 IC50 = 2690.0 nM 5.57
CHEMBL5436270 IC50 = 3640.0 nM 5.44
CHEMBL5421285 IC50 = 3740.0 nM 5.43
CHEMBL2018554 IC50 = 3840.0 nM 5.42
CHEMBL5400279 IC50 = 4000.0 nM 5.40
CHEMBL5418340 IC50 = 4270.0 nM 5.37
CHEMBL5422642 IC50 = 6130.0 nM 5.21