Assay Detail
Binding
CHEMBL5355272
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of human BChE using butyrylthiocholine as substrate preincubated for 5 mins followed by substrate addition and measured after 2 mins by Perkin elmer based spectrophotometer analysis
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Novel slow-binding reversible acetylcholinesterase inhibitors based on uracil moieties for possible treatment of myasthenia gravis and protection from organophosphate poisoning.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 9 | 4.89 | 6.00 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL812 | PYRIDOSTIGMINE BROMIDE | 4.0 | 1 | 6.00 |
| CHEMBL5396497 | — | — | 1 | 5.40 |
| CHEMBL5417346 | — | — | 1 | 5.22 |
| CHEMBL5434553 | — | — | 1 | 4.60 |
| CHEMBL5440753 | — | — | 1 | 4.40 |
| CHEMBL5419243 | — | — | 1 | 4.40 |
| CHEMBL5426103 | — | — | 1 | 4.22 |
| CHEMBL5418455 | — | — | 1 | - |
| CHEMBL5417632 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL812 | PYRIDOSTIGMINE BROMIDE | IC50 | = | 1000.0 | nM | 6.00 |
| CHEMBL5396497 | — | IC50 | = | 4000.0 | nM | 5.40 |
| CHEMBL5417346 | — | IC50 | = | 6000.0 | nM | 5.22 |
| CHEMBL5434553 | — | IC50 | = | 25000.0 | nM | 4.60 |
| CHEMBL5440753 | — | IC50 | = | 40000.0 | nM | 4.40 |
| CHEMBL5419243 | — | IC50 | = | 40000.0 | nM | 4.40 |
| CHEMBL5426103 | — | IC50 | = | 60000.0 | nM | 4.22 |
| CHEMBL5418455 | — | IC50 | = | 440000.0 | nM | - |
| CHEMBL5417632 | — | IC50 | = | 200000.0 | nM | - |