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Assay Detail

CHEMBL5355535

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at human beta1-adrenergic receptor expressed in CHO-K1 cells co-expressing CRE-SPAP reporter gene assessed as inhibition of fenoterol-induced CRE-SPAP production by measuring rightward shift of the fenoterol concentration response curve measured after 5 hrs incubation
34
Total Activities
34
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type CHO-K1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Beta-1 adrenergic receptor (CHEMBL213)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Rational design, synthesis, and pharmacological evaluation of a cohort of novel beta-adrenergic receptors ligands enables an assessment of structure-activity relationships.
Tricomi J, Landini L, Nieddu V, Cavallaro U, Baker JG, Papakyriakou A, Richichi B.
Eur J Med Chem (2023) 246 : 114961 -114961
PubMed 36495629 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 34 6.11 8.99

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL280822 CGP-20712A 1 8.99
CHEMBL1326499 1 7.43
CHEMBL5427527 1 6.99
CHEMBL513389 ZENIDOLOL 2.0 1 6.92
CHEMBL5397120 1 6.87
CHEMBL5430355 1 6.59
CHEMBL4238084 1 6.47
CHEMBL5415148 1 5.80
CHEMBL5428413 1 5.78
CHEMBL5416996 1 5.76
CHEMBL5431834 1 5.64
CHEMBL5422259 1 5.62
CHEMBL5395772 1 5.51
CHEMBL5432691 1 5.50
CHEMBL5437540 1 5.49
CHEMBL5423415 1 5.44
CHEMBL5432967 1 5.44
CHEMBL5426087 1 5.41
CHEMBL5430136 1 5.25
CHEMBL5402516 1 5.22
CHEMBL5434276 1 -
CHEMBL5395675 1 -
CHEMBL5416424 1 -
CHEMBL5413563 1 -
CHEMBL5403431 1 -
CHEMBL5395432 1 -
CHEMBL5429806 1 -
CHEMBL5409581 1 -
CHEMBL5399055 1 -
CHEMBL5413817 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL280822 CGP-20712A Kd = 1.023 nM 8.99
CHEMBL1326499 Kd = 37.15 nM 7.43
CHEMBL5427527 Kd = 102.33 nM 6.99
CHEMBL513389 ZENIDOLOL Kd = 120.23 nM 6.92
CHEMBL5397120 Kd = 134.9 nM 6.87
CHEMBL5430355 Kd = 257.04 nM 6.59
CHEMBL4238084 Kd = 338.84 nM 6.47
CHEMBL5415148 Kd = 1584.89 nM 5.80
CHEMBL5428413 Kd = 1659.59 nM 5.78
CHEMBL5416996 Kd = 1737.8 nM 5.76
CHEMBL5431834 Kd = 2290.87 nM 5.64
CHEMBL5422259 Kd = 2398.83 nM 5.62
CHEMBL5395772 Kd = 3090.3 nM 5.51
CHEMBL5432691 Kd = 3162.28 nM 5.50
CHEMBL5437540 Kd = 3235.94 nM 5.49
CHEMBL5423415 Kd = 3630.78 nM 5.44
CHEMBL5432967 Kd = 3630.78 nM 5.44
CHEMBL5426087 Kd = 3890.45 nM 5.41
CHEMBL5430136 Kd = 5623.41 nM 5.25
CHEMBL5402516 Kd = 6025.6 nM 5.22
CHEMBL5434276 Kd > 10000.0 nM -
CHEMBL5395675 Kd < 0.1 nM -
CHEMBL5416424 Kd > 10000.0 nM -
CHEMBL5413563 Kd > 10000.0 nM -
CHEMBL5403431 Kd > 10000.0 nM -
CHEMBL5395432 Kd > 10000.0 nM -
CHEMBL5429806 Kd > 10000.0 nM -
CHEMBL5409581 Kd > 10000.0 nM -
CHEMBL5399055 Kd > 10000.0 nM -
CHEMBL5413817 Kd > 10000.0 nM -
CHEMBL5400977 Kd > 10000.0 nM -
CHEMBL5417897 Kd > 10000.0 nM -
CHEMBL5422894 Kd > 10000.0 nM -
CHEMBL5410388 Kd > 10000.0 nM -