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Assay Detail

CHEMBL5357834

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of porcine PDHc E1-subunit incubated for 30 mins followed by pyruvate addition in presence of thiamine pyrophosphate by microplate reader analysis
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Sus scrofa
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Thiamine analogues featuring amino-oxetanes as potent and selective inhibitors of pyruvate dehydrogenase.
Chan AHY, Ho TCS, Leeper FJ.
Bioorg Med Chem Lett (2024) 98 : 129571 -129571
PubMed 38036274 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 5.19 6.05

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5419102 1 6.05
CHEMBL5417802 1 6.00
CHEMBL5417002 1 5.60
CHEMBL5410728 1 5.52
CHEMBL5419964 1 5.48
CHEMBL5420203 1 5.46
CHEMBL401772 3-DEAZATHIAMINE 1 5.44
CHEMBL5440127 1 5.29
CHEMBL5408769 1 5.20
CHEMBL5417344 1 5.11
CHEMBL5401064 1 5.04
CHEMBL5173431 1 4.97
CHEMBL5437447 1 4.68
CHEMBL4277786 1 4.52
CHEMBL5406774 1 4.46
CHEMBL5441074 1 4.18

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5419102 IC50 = 890.0 nM 6.05
CHEMBL5417802 IC50 = 990.0 nM 6.00
CHEMBL5417002 IC50 = 2500.0 nM 5.60
CHEMBL5410728 IC50 = 3000.0 nM 5.52
CHEMBL5419964 IC50 = 3300.0 nM 5.48
CHEMBL5420203 IC50 = 3500.0 nM 5.46
CHEMBL401772 3-DEAZATHIAMINE IC50 = 3600.0 nM 5.44
CHEMBL5440127 IC50 = 5100.0 nM 5.29
CHEMBL5408769 IC50 = 6300.0 nM 5.20
CHEMBL5417344 IC50 = 7800.0 nM 5.11
CHEMBL5401064 IC50 = 9200.0 nM 5.04
CHEMBL5173431 IC50 = 10600.0 nM 4.97
CHEMBL5437447 IC50 = 21000.0 nM 4.68
CHEMBL4277786 IC50 = 30000.0 nM 4.52
CHEMBL5406774 IC50 = 35000.0 nM 4.46
CHEMBL5441074 IC50 = 66000.0 nM 4.18