Assay Detail
Functional
CHEMBL5359161
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Partial agonist activity at human 5-HT2BR expressed in human Flp-In-T-REx-293 cells assessed as increase in calcium flux incubated for 60 mins followed by incubation for 30 mins in dark by Flou-4 dye based FLIPR assay
8
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Homo sapiens |
| Cell Type | Flp-In-T-REx-293 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Identification of Spiro[chromene-2,4'-piperidine]s as Potent, Selective, and G<sub>q</sub>-Biased 5-HT<sub>2C</sub> Receptor Partial Agonists.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 8 | 7.11 | 8.92 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL39 | SEROTONIN | 3.0 | 2 | 8.92 |
| CHEMBL5416800 | — | — | 2 | 7.20 |
| CHEMBL5396618 | — | — | 2 | 6.33 |
| CHEMBL4913232 | — | — | 2 | 6.05 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL39 | SEROTONIN | EC50 | = | 1.19 | nM | 8.92 |
| CHEMBL39 | SEROTONIN | EC50 | = | 1.259 | nM | 8.90 |
| CHEMBL5416800 | — | EC50 | = | 63.1 | nM | 7.20 |
| CHEMBL5416800 | — | EC50 | = | 68.4 | nM | 7.17 |
| CHEMBL5396618 | — | EC50 | = | 469.7 | nM | 6.33 |
| CHEMBL5396618 | — | EC50 | = | 501.19 | nM | 6.30 |
| CHEMBL4913232 | — | EC50 | = | 897.0 | nM | 6.05 |
| CHEMBL4913232 | — | EC50 | = | 1000.0 | nM | 6.00 |