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Assay Detail

CHEMBL5359219

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Plasmodium falciparum Threonyl tRNA synthetase expressed in Escherichia coli Rossetta 2 (DE3) assessed as apparent inhibition constant by measuring formation of Thr-tRNA Thr using [14C]threonine and unlabeled threonine by scintillation counting analysis
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Plasmodium falciparum
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

threonine--tRNA ligase (CHEMBL5465377)
Type SINGLE PROTEIN
Organism Plasmodium falciparum

Publication

Discovery of Malarial Threonyl tRNA Synthetase Inhibitors by Screening of a Focused Fragment Library.
Bolsakova J, Bobrovs R, Varacheva L, Rudnickiha A, Kanepe I, Parisini E, Jirgensons A.
ACS Med Chem Lett (2024) 15 : 76 -80
PubMed 38229753 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 1 6.80 6.80

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL70590 BORRELIDIN 1 6.80

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL70590 BORRELIDIN Ki = 160.0 nM 6.80