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Assay Detail

CHEMBL5360778

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human DDAH1 expressed in HEK293 cells using ADMA as substrate preincubated for 5 mins
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 (CHEMBL6036)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Chemical similarities and differences among inhibitors of nitric oxide synthase, arginase and dimethylarginine dimethylaminohydrolase-1: Implications for the design of novel enzyme inhibitors modulating the nitric oxide pathway.
Doman AJ, Tommasi S, Perkins MV, McKinnon RA, Mangoni AA, Nair PC.
Bioorg Med Chem (2022) 72 : 116970 -116970
PubMed 36063653 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 4.91 5.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4444007 1 5.52
CHEMBL5437726 1 4.75
CHEMBL5410984 1 4.47
CHEMBL5438148 1 -
CHEMBL5434286 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4444007 IC50 = 3000.0 nM 5.52
CHEMBL5437726 IC50 = 18000.0 nM 4.75
CHEMBL5410984 IC50 = 34000.0 nM 4.47
CHEMBL5438148 IC50 = 230000.0 nM -
CHEMBL5434286 IC50 = 131000.0 nM -