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Assay Detail

CHEMBL5363875

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Partial agonist activity at Rluc8 fused mouse MOR transfected in HEK293T cells coexpressing wild type GRK2 assessed as arrestin-3 recruitment using coelenterazine as substrate incubated for 40 mins by BRET assay
15
Total Activities
10
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type HEK293T
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Mu-type opioid receptor (CHEMBL2858)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Pharmacological and Physicochemical Properties Optimization for Dual-Target Dopamine D<sub>3</sub> (D<sub>3</sub>R) and μ-Opioid (MOR) Receptor Ligands as Potentially Safer Analgesics.
Bonifazi A, Saab E, Sanchez J, Nazarova AL, Zaidi SA, Jahan K, Katritch V, Canals M, Lane JR, Newman AH.
J Med Chem (2023) 66 : 10304 -10341
PubMed 37467430 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 10 6.39 7.49
Activity 5 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2443262 OLICERIDINE 4.0 2 7.49
CHEMBL70 MORPHINE 4.0 2 6.68
CHEMBL5418819 2 6.33
CHEMBL5412995 2 6.06
CHEMBL5397609 2 5.38
CHEMBL5432993 1 -
CHEMBL5434650 1 -
CHEMBL5414796 1 -
CHEMBL5404724 1 -
CHEMBL5395539 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2443262 OLICERIDINE EC50 = 32.36 nM 7.49
CHEMBL2443262 OLICERIDINE EC50 = 32.5 nM 7.49
CHEMBL70 MORPHINE EC50 = 208.93 nM 6.68
CHEMBL70 MORPHINE EC50 = 207.0 nM 6.68
CHEMBL5418819 EC50 = 467.74 nM 6.33
CHEMBL5418819 EC50 = 470.0 nM 6.33
CHEMBL5412995 EC50 = 870.96 nM 6.06
CHEMBL5412995 EC50 = 876.0 nM 6.06
CHEMBL5397609 EC50 = 4168.69 nM 5.38
CHEMBL5397609 EC50 = 4206.0 nM 5.38
CHEMBL5432993 Activity - -
CHEMBL5434650 Activity - -
CHEMBL5414796 Activity - -
CHEMBL5404724 Activity - -
CHEMBL5395539 Activity - -