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Assay Detail

CHEMBL5363877

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Agonist activity at Rluc8 fused mouse MOR transfected in HEK293T cells coexpressing wild type GRK2 assessed as arrestin-3 recruitment using coelenterazine as substrate incubated for 40 mins by BRET assay
6
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type HEK293T
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Mu-type opioid receptor (CHEMBL2858)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Pharmacological and Physicochemical Properties Optimization for Dual-Target Dopamine D<sub>3</sub> (D<sub>3</sub>R) and μ-Opioid (MOR) Receptor Ligands as Potentially Safer Analgesics.
Bonifazi A, Saab E, Sanchez J, Nazarova AL, Zaidi SA, Jahan K, Katritch V, Canals M, Lane JR, Newman AH.
J Med Chem (2023) 66 : 10304 -10341
PubMed 37467430 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 6 6.34 7.26

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL38874 DAMGO 2 7.26
CHEMBL5433458 2 6.58
CHEMBL5399270 2 5.19

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL38874 DAMGO EC50 = 54.95 nM 7.26
CHEMBL38874 DAMGO EC50 = 54.4 nM 7.26
CHEMBL5433458 EC50 = 263.03 nM 6.58
CHEMBL5433458 EC50 = 261.0 nM 6.58
CHEMBL5399270 EC50 = 6456.54 nM 5.19
CHEMBL5399270 EC50 = 6478.0 nM 5.19