Assay Detail
Binding
CHEMBL5364211
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Binding affinity to full-length wild-type human NNMT (1 to 270 residues) expressed in Escherichia coli BL21(DE3) cells assessed as apparent inhibition constant preincubated for 30 mins followed by nicotinamide addition by fluorescence-based SAHH-coupled assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Structure-Activity Relationship Studies on Cell-Potent Nicotinamide <i>N</i>-Methyltransferase Bisubstrate Inhibitors.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 3 | 8.65 | 8.92 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5408922 | — | — | 1 | 8.92 |
| CHEMBL5433732 | — | — | 1 | 8.80 |
| CHEMBL5420435 | — | — | 1 | 8.23 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5408922 | — | Ki | = | 1.2 | nM | 8.92 |
| CHEMBL5433732 | — | Ki | = | 1.6 | nM | 8.80 |
| CHEMBL5420435 | — | Ki | = | 5.9 | nM | 8.23 |