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Assay Detail

CHEMBL5365107

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antitumor activity against CHO cells expressing PCFT (R2/PCFT4) transporter assessed as inhibition of cell growth measured upto 96 hrs by Cell titer blue assay
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Cricetulus griseus
Cell Type CHO
Confidence 1 — Organism
Curated By Autocuration

Target

CHO (CHEMBL613853)
Type CELL-LINE
Organism Cricetulus griseus

Publication

Structure-Based Design of Transport-Specific Multitargeted One-Carbon Metabolism Inhibitors in Cytosol and Mitochondria.
Nayeen MJ, Katinas JM, Magdum T, Shah K, Wong JE, O'Connor CE, Fifer AN, Wallace-Povirk A, Hou Z, Matherly LH, Dann CE, Gangjee A.
J Med Chem (2023) 66 : 11294 -11323
PubMed 37582241 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 6.77 7.88

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL225072 PEMETREXED 4.0 1 7.88
CHEMBL5408906 1 7.01
CHEMBL5431881 1 6.72
CHEMBL5432700 1 6.37
CHEMBL5405010 1 6.32
CHEMBL5418034 1 6.30
CHEMBL5417651 1 -
CHEMBL5438587 1 -
CHEMBL5440815 1 -
CHEMBL5421259 1 -
CHEMBL5402700 1 -
CHEMBL5429283 1 -
CHEMBL5427804 1 -
CHEMBL5431331 1 -
CHEMBL5415985 1 -
CHEMBL5400671 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL225072 PEMETREXED IC50 = 13.2 nM 7.88
CHEMBL5408906 IC50 = 98.05 nM 7.01
CHEMBL5431881 IC50 = 191.4 nM 6.72
CHEMBL5432700 IC50 = 431.0 nM 6.37
CHEMBL5405010 IC50 = 479.17 nM 6.32
CHEMBL5418034 IC50 = 497.07 nM 6.30
CHEMBL5417651 IC50 > 1000.0 nM -
CHEMBL5438587 IC50 > 1000.0 nM -
CHEMBL5440815 IC50 > 1000.0 nM -
CHEMBL5421259 IC50 > 1000.0 nM -
CHEMBL5402700 IC50 > 1000.0 nM -
CHEMBL5429283 IC50 > 1000.0 nM -
CHEMBL5427804 IC50 > 1000.0 nM -
CHEMBL5431331 IC50 > 1000.0 nM -
CHEMBL5415985 IC50 > 1000.0 nM -
CHEMBL5400671 IC50 > 1000.0 nM -