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Assay Detail

CHEMBL5365709

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of TYK2 JH2 domain in human whole blood assessed as reduction in IFNalpha-induced STAT5 phosphorylation preincubated for 1 hr followed by IFNalpha stimulation and measured after 15 mins by fluorescence assay
27
Total Activities
27
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Tissue Blood
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Non-receptor tyrosine-protein kinase TYK2 (CHEMBL3553)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-activity relationship study of central pyridine-derived TYK2 JH2 inhibitors: Optimization of the PK profile through C4' and C6 variations.
Xiao Z, Yang MG, Liu C, Sherwood T, Gilmore JL, Lin J, Li P, Wu DR, Tokarski J, Li S, Cheng L, Xie C, Fan J, Dierks E, Strnad J, Cvijic ME, Khan J, Ruzanov M, Galella M, Khandelwal P, Dyckman AJ, Mathur A, Lombardo LJ, Macor JE, Carter PH, Aranibar N, Burke JR, Weinstein DS.
Bioorg Med Chem Lett (2023) 91 : 129373 -129373
PubMed 37315697 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 27 7.07 7.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5413544 1 7.96
CHEMBL5439728 1 7.82
CHEMBL5437122 1 7.80
CHEMBL5411149 1 7.66
CHEMBL5412034 1 7.55
CHEMBL5403115 1 7.48
CHEMBL5408060 1 7.42
CHEMBL5427107 1 7.39
CHEMBL5437552 1 7.39
CHEMBL5409590 1 7.28
CHEMBL5420187 1 7.28
CHEMBL5433636 1 7.22
CHEMBL5415132 1 7.13
CHEMBL5403477 1 7.12
CHEMBL5396654 1 7.03
CHEMBL5440225 1 7.01
CHEMBL5438688 1 6.97
CHEMBL5430970 1 6.91
CHEMBL5418485 1 6.88
CHEMBL5405178 1 6.88
CHEMBL5399546 1 6.76
CHEMBL5401385 1 6.54
CHEMBL5406364 1 6.53
CHEMBL5411146 1 6.51
CHEMBL5403572 1 6.47
CHEMBL5409445 1 6.31
CHEMBL5416323 1 5.58

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5413544 IC50 = 11.0 nM 7.96
CHEMBL5439728 IC50 = 15.0 nM 7.82
CHEMBL5437122 IC50 = 16.0 nM 7.80
CHEMBL5411149 IC50 = 22.0 nM 7.66
CHEMBL5412034 IC50 = 28.0 nM 7.55
CHEMBL5403115 IC50 = 33.0 nM 7.48
CHEMBL5408060 IC50 = 38.0 nM 7.42
CHEMBL5427107 IC50 = 41.0 nM 7.39
CHEMBL5437552 IC50 = 41.0 nM 7.39
CHEMBL5409590 IC50 = 52.0 nM 7.28
CHEMBL5420187 IC50 = 52.0 nM 7.28
CHEMBL5433636 IC50 = 60.0 nM 7.22
CHEMBL5415132 IC50 = 74.0 nM 7.13
CHEMBL5403477 IC50 = 76.0 nM 7.12
CHEMBL5396654 IC50 = 94.0 nM 7.03
CHEMBL5440225 IC50 = 97.0 nM 7.01
CHEMBL5438688 IC50 = 108.0 nM 6.97
CHEMBL5430970 IC50 = 124.0 nM 6.91
CHEMBL5418485 IC50 = 131.0 nM 6.88
CHEMBL5405178 IC50 = 131.0 nM 6.88
CHEMBL5399546 IC50 = 175.0 nM 6.76
CHEMBL5401385 IC50 = 288.0 nM 6.54
CHEMBL5406364 IC50 = 296.0 nM 6.53
CHEMBL5411146 IC50 = 312.0 nM 6.51
CHEMBL5403572 IC50 = 339.0 nM 6.47
CHEMBL5409445 IC50 = 485.0 nM 6.31
CHEMBL5416323 IC50 = 2610.0 nM 5.58