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Assay Detail

CHEMBL5367046

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Competitive inhibition of NOX2 (unknown origin) expressed in HEK293T cells assessed as inhibition of PMA-induced hydrogen peroxide production pretreated for 15 mins followed by PMA stimulation and measured after 10 mins by Amplex red based fluorescence based analysis
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293T
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cytochrome b-245 heavy chain (CHEMBL1287627)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

NADPH Oxidases: From Molecular Mechanisms to Current Inhibitors.
Cipriano A, Viviano M, Feoli A, Milite C, Sarno G, Castellano S, Sbardella G.
J Med Chem (2023) 66 : 11632 -11655
PubMed 37650225 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 5.50 5.50

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5424942 1 5.50

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5424942 IC50 = 3162.28 nM 5.50