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Assay Detail

CHEMBL5369311

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of MCP-1 in LPS-stimulated human PBMC cells pretreated with compound for 30 mins followed by LPS-stimulation and measured after 10 mins by microplate analysis
16
Total Activities
16
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type PBMC
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

C-C motif chemokine 2 (CHEMBL1649052)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design and Characterization of 1,3-Dihydro-2<i>H</i>-benzo[<i>d</i>]azepin-2-ones as Rule-of-5 Compliant Bivalent BET Inhibitors.
Bamborough P, Chung CW, Goodwin NC, Mitchell DJ, Neipp CE, Phillipou A, Preston A, Prinjha RK, Soden PE, Watson RJ, Demont EH.
ACS Med Chem Lett (2023) 14 : 1231 -1236
PubMed 37736196 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 8.11 10.40
pIC50 2 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5426022 1 10.40
CHEMBL5422047 1 9.60
CHEMBL4078100 AZD-5153 1.0 1 8.50
CHEMBL1957266 1 8.20
CHEMBL4438171 1 8.10
CHEMBL4636881 GSK046 1 7.50
CHEMBL2017291 1 7.40
CHEMBL4639128 GSK778 1 7.40
CHEMBL5220623 1 7.30
CHEMBL4519518 1 6.70
CHEMBL5426762 1 -
CHEMBL5403564 1 -
CHEMBL5408181 1 -
CHEMBL5427202 1 -
CHEMBL5417286 1 -
CHEMBL5433432 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5426022 IC50 = 0.03981 nM 10.40
CHEMBL5422047 IC50 = 0.2512 nM 9.60
CHEMBL4078100 AZD-5153 IC50 = 3.162 nM 8.50
CHEMBL1957266 IC50 = 6.31 nM 8.20
CHEMBL4438171 IC50 = 7.943 nM 8.10
CHEMBL4636881 GSK046 IC50 = 31.62 nM 7.50
CHEMBL2017291 IC50 = 39.81 nM 7.40
CHEMBL4639128 GSK778 IC50 = 39.81 nM 7.40
CHEMBL5220623 IC50 = 50.12 nM 7.30
CHEMBL4519518 IC50 = 199.53 nM 6.70
CHEMBL5426762 IC50 < 1.995 nM -
CHEMBL5403564 IC50 < 1.995 nM -
CHEMBL5408181 IC50 < 1.995 nM -
CHEMBL5427202 pIC50 - -
CHEMBL5417286 pIC50 - -
CHEMBL5433432 IC50 < 0.1995 nM -