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Assay Detail

CHEMBL5370508

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of SARS-CoV-2 main protease expressed in Escherichia coli using [NH2-C(EDANS)VNSTQSGLRK(DABCYL)M-COOH] FRET peptide as substrate pre-incubated for 20 mins followed by substrate addition enzyme activity measured every 5 mins for 120 mins by fluorescence assay
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Severe acute respiratory syndrome coronavirus 2
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Replicase polyprotein 1ab (CHEMBL4523582)
Type SINGLE PROTEIN
Organism Severe acute respiratory syndrome coronavirus 2

Publication

Design and Optimization of Novel Competitive, Non-peptidic, SARS-CoV-2 M<sup>pro</sup> Inhibitors.
Jacobs L, van der Westhuyzen A, Pribut N, Dentmon ZW, Cui D, D'Erasmo MP, Bartsch PW, Liu K, Cox RM, Greenlund SF, Plemper RK, Mitchell D, Marlow J, Andrews MK, Krueger RE, Sticher ZM, Kolykhalov AA, Natchus MG, Zhou B, Pelly SC, Liotta DC.
ACS Med Chem Lett (2023) 14 : 1434 -1440
PubMed 37849558 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 7.30 8.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5431872 1 8.22
CHEMBL5428134 1 8.05
CHEMBL5406180 1 7.92
CHEMBL5427542 1 7.92
CHEMBL5399537 1 7.89
CHEMBL5394734 1 7.80
CHEMBL5412903 1 7.77
CHEMBL5439438 1 7.68
CHEMBL4867191 1 7.55
CHEMBL5423178 1 7.37
CHEMBL4861071 1 7.36
CHEMBL5402360 1 7.26
CHEMBL5420763 1 7.00
CHEMBL5437439 1 6.92
CHEMBL5434245 1 6.55
CHEMBL5404766 1 5.80
CHEMBL5405138 1 5.00
CHEMBL1214124 PERAMPANEL 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5431872 IC50 = 6.0 nM 8.22
CHEMBL5428134 IC50 = 9.0 nM 8.05
CHEMBL5406180 IC50 = 12.0 nM 7.92
CHEMBL5427542 IC50 = 12.0 nM 7.92
CHEMBL5399537 IC50 = 13.0 nM 7.89
CHEMBL5394734 IC50 = 16.0 nM 7.80
CHEMBL5412903 IC50 = 17.0 nM 7.77
CHEMBL5439438 IC50 = 21.0 nM 7.68
CHEMBL4867191 IC50 = 28.0 nM 7.55
CHEMBL5423178 IC50 = 43.0 nM 7.37
CHEMBL4861071 IC50 = 44.0 nM 7.36
CHEMBL5402360 IC50 = 55.0 nM 7.26
CHEMBL5420763 IC50 = 100.0 nM 7.00
CHEMBL5437439 IC50 = 120.0 nM 6.92
CHEMBL5434245 IC50 = 280.0 nM 6.55
CHEMBL5404766 IC50 = 1590.0 nM 5.80
CHEMBL5405138 IC50 = 9990.0 nM 5.00
CHEMBL1214124 PERAMPANEL IC50 ~ 100000.0 nM -