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Assay Detail

CHEMBL5372317

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human GUSbeta using 4-MUG as substrate pre-incubated for 10 mins followed by substrate addition and measured after 60 mins by fluorescence based assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Beta-glucuronidase (CHEMBL2728)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-based lead optimization to improve potency and selectivity of a novel tetrahydroimidazo[1,2-a]pyridine-5-carboxylic acid series of heparanase-1 inhibitor.
Imai Y, Suzuki R, Wakasugi D, Matsuda D, Tanaka-Yamamoto N, Ohki Y, Mima M, Endo M, Tabata R, Matsuzawa H, Hasegawa Y, Kato S, Sugisaki M, Miyagawa H, Fujimoto N, Fukunaga T, Kato S, Takahashi T, Kakinuma H.
Bioorg Med Chem (2023) 93 : 117460 -117460
PubMed 37660465 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 7.09 8.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5409470 1 8.10
CHEMBL5404984 1 6.08

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5409470 IC50 = 8.0 nM 8.10
CHEMBL5404984 IC50 = 839.0 nM 6.08