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Assay Detail

CHEMBL5373955

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Agonist activity at mouse TLR4 in human HEK-Blue mTLR4 cells assessed as NF-kappaB activation incubated for 48 hrs by SEAP reporter gene based Quanti-blue assay
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Cell Type HEK-Blue mTLR4
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Toll-like receptor 4 (CHEMBL1795167)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Diamino Allose Phosphates: Novel, Potent, and Highly Stable Toll-like Receptor 4 Agonists.
Khalaf JK, Bess LS, Walsh LM, Ward JM, Johnson CL, Livesay MT, Jackson KJ, Evans JT, Ryter KT, Bazin-Lee HG.
J Med Chem (2023) 66 : 13900 -13917
PubMed 37847244 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 10 9.16 9.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5407900 1 9.82
CHEMBL5423611 1 9.72
CHEMBL5432710 1 9.43
CHEMBL5428754 1 9.37
CHEMBL5433813 1 9.30
CHEMBL5407887 1 9.17
CHEMBL5417929 1 8.85
CHEMBL5422378 1 8.58
CHEMBL5434803 1 8.18
CHEMBL507938 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5407900 EC50 = 0.15 nM 9.82
CHEMBL5423611 EC50 = 0.19 nM 9.72
CHEMBL5432710 EC50 = 0.37 nM 9.43
CHEMBL5428754 EC50 = 0.43 nM 9.37
CHEMBL5433813 EC50 = 0.5 nM 9.30
CHEMBL5407887 EC50 = 0.68 nM 9.17
CHEMBL5417929 EC50 = 1.4 nM 8.85
CHEMBL5422378 EC50 = 2.64 nM 8.58
CHEMBL5434803 EC50 = 6.54 nM 8.18
CHEMBL507938 EC50 = 0.005 nM -