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Assay Detail

CHEMBL5375221

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Total binding affinity to N-terminal 3xHA/Nluc-tagged full-length wild-type human GPR3 expressed in HEK293A cells using furimazine as substrate measured for 3 hrs by NanoBRET assay
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293-A
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

G-protein coupled receptor 3 (CHEMBL4523856)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Development of Fluorescent AF64394 Analogues Enables Real-Time Binding Studies for the Orphan Class A GPCR GPR3.
Bresinsky M, Shahraki A, Kolb P, Pockes S, Schihada H.
J Med Chem (2023) 66 : 15025 -15041
PubMed 37907069 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 11 5.43 6.05

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5410506 1 6.05
CHEMBL5405597 1 5.47
CHEMBL5423734 1 5.35
CHEMBL5398023 1 5.35
CHEMBL5394725 1 4.95
CHEMBL5414539 1 -
CHEMBL5420748 1 -
CHEMBL5401699 1 -
CHEMBL5427163 1 -
CHEMBL5396623 1 -
CHEMBL5421688 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5410506 EC50 = 891.25 nM 6.05
CHEMBL5405597 EC50 = 3388.44 nM 5.47
CHEMBL5423734 EC50 = 4466.84 nM 5.35
CHEMBL5398023 EC50 = 4466.84 nM 5.35
CHEMBL5394725 EC50 = 11220.18 nM 4.95
CHEMBL5414539 EC50 > 10000.0 nM -
CHEMBL5420748 EC50 > 10000.0 nM -
CHEMBL5401699 EC50 > 31622.78 nM -
CHEMBL5427163 EC50 > 31622.78 nM -
CHEMBL5396623 EC50 > 31622.78 nM -
CHEMBL5421688 EC50 > 31622.78 nM -