Assay Detail
Functional
CHEMBL5375231
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Agonist activity at FLAG/GFP-tagged GPR3 (unknown origin)-mediated cAMP signaling in HEK293 cells using d2-cAMP as substrate preincubated for 30 mins followed by substrate addition measured after 60 mins by HTRF assay
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Homo sapiens |
| Cell Type | HEK293 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Development of Fluorescent AF64394 Analogues Enables Real-Time Binding Studies for the Orphan Class A GPCR GPR3.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 1 | 6.00 | 6.00 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL397686 | DIPHENYLENEIODONIUM CHLORIDE | — | 1 | 6.00 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL397686 | DIPHENYLENEIODONIUM CHLORIDE | EC50 | = | 1000.0 | nM | 6.00 |