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Assay Detail

CHEMBL5377103

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Modulator activity at wild type RyR2 (unknown origin) expressed in HEK293T co-expressed with R-CEPIA1er assessed as decrease in RyR2-mediated calcium leak in presence of calcium chloride by time-lapse fluorescence analysis
10
Total Activities
10
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293T
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Ryanodine receptor 2 (CHEMBL4403)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

1,4-Benzothiazepines with Cyclopropanol Groups and Their Structural Analogues Exhibit Both RyR2-Stabilizing and SERCA2a-Stimulating Activities.
Mitronova GY, Quentin C, Belov VN, Wegener JW, Kiszka KA, Lehnart SE.
J Med Chem (2023) 66 : 15761 -15775
PubMed 37991191 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 6 6.69 7.99
Activity 4 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5413930 1 7.99
CHEMBL5409619 1 7.73
CHEMBL5398491 1 7.09
CHEMBL5435124 1 5.57
CHEMBL5437537 1 5.09
CHEMBL2104951 ALADORIAN 2.0 1 -
CHEMBL2440854 1 -
CHEMBL4594418 SURLORIAN 2.0 1 -
CHEMBL5409315 1 -
CHEMBL5426069 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5413930 EC50 = 10.2 nM 7.99
CHEMBL5409619 EC50 = 18.6 nM 7.73
CHEMBL5398491 EC50 = 81.5 nM 7.09
CHEMBL5435124 EC50 = 2700.0 nM 5.57
CHEMBL5437537 EC50 = 8170.0 nM 5.09
CHEMBL2104951 ALADORIAN Activity - -
CHEMBL2440854 Activity - -
CHEMBL4594418 SURLORIAN Activity - -
CHEMBL5409315 Activity - -
CHEMBL5426069 EC50 > 23000.0 nM -