Assay Detail
Binding
CHEMBL5378688
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of full length recombinant human HDAC3 expressed in Sf9 cells using 6-carboxyfluorescein peptide as substrate preincubated with compound for 6 hrs followed by substrate addition and measured after 3 hrs by electrophoretic mobility shift assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | Sf9 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Discovery of YSR734: A Covalent HDAC Inhibitor with Cellular Activity in Acute Myeloid Leukemia and Duchenne Muscular Dystrophy.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 3 | 7.25 | 7.46 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL27759 | ENTINOSTAT | 3.0 | 1 | 7.46 |
| CHEMBL5412725 | — | — | 1 | 7.44 |
| CHEMBL5411315 | — | — | 1 | 6.84 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL27759 | ENTINOSTAT | IC50 | = | 35.0 | nM | 7.46 |
| CHEMBL5412725 | — | IC50 | = | 36.0 | nM | 7.44 |
| CHEMBL5411315 | — | IC50 | = | 143.0 | nM | 6.84 |