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Assay Detail

CHEMBL5378753

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Toxicity
Antiproliferative activity against HEK293 cells harboring p53 assessed inhibition of cell growth incubated for 4 days by CCK-8 assay
3
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Toxicity
Organism Homo sapiens
Cell Type HEK293
Confidence 1 — Organism
Curated By Autocuration

Target

HEK293 (CHEMBL614818)
Type CELL-LINE
Organism Homo sapiens

Publication

Discovery of JN122, a Spiroindoline-Containing Molecule that Inhibits MDM2/p53 Protein-Protein Interaction and Exerts Robust In Vivo Antitumor Efficacy.
Cheng J, Yan Z, Jiang K, Liu C, Xu D, Lyu X, Hu X, Zhang S, Zhou Y, Li J, Zhao Y.
J Med Chem (2023) 66 : 16991 -17025
PubMed 38062557 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 5.35 5.44

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5397752 1 5.44
CHEMBL5425989 2 5.37

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5397752 IC50 = 3620.0 nM 5.44
CHEMBL5425989 IC50 = 4280.0 nM 5.37
CHEMBL5425989 IC50 = 5940.0 nM 5.23