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Assay Detail

CHEMBL5384758

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of His tagged human recombinant CDK8/Cyclin C expressed in baculovirus expression system incubated for 15 mins by FRET-based LanthaScreen binding competition assay
24
Total Activities
24
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

CDK8/Cyclin C (CHEMBL3038474)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Discovery of Potent, Selective, and Orally Bioavailable Small-Molecule Inhibitors of CDK8 for the Treatment of Cancer.
Li Y, Liu Y, Wu J, Liu X, Wang L, Wang J, Yu J, Qi H, Qin L, Ding X, Ren F, Zhavoronkov A.
J Med Chem (2023) 66 : 5439 -5452
PubMed 37029334 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 24 8.50 9.43

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5435821 1 9.43
CHEMBL5434183 1 9.40
CHEMBL5409410 1 9.40
CHEMBL5405004 1 9.32
CHEMBL5414359 1 9.31
CHEMBL5416117 1 9.20
CHEMBL5396725 1 9.15
CHEMBL5402477 1 9.15
CHEMBL5411825 1 9.08
CHEMBL5440319 1 9.06
CHEMBL5416758 1 9.05
CHEMBL5425043 1 8.82
CHEMBL5423457 1 8.48
CHEMBL5429467 1 8.32
CHEMBL5417604 1 8.27
CHEMBL5432423 1 8.21
CHEMBL5427899 1 8.13
CHEMBL4225966 SEL-120 FREE BASE 1.0 1 7.85
CHEMBL5416134 1 7.77
CHEMBL5412471 1 7.45
CHEMBL5397969 1 7.10
CHEMBL5436530 1 7.08
CHEMBL5402190 1 6.56
CHEMBL5406025 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5435821 IC50 = 0.37 nM 9.43
CHEMBL5434183 IC50 = 0.4 nM 9.40
CHEMBL5409410 IC50 = 0.4 nM 9.40
CHEMBL5405004 IC50 = 0.48 nM 9.32
CHEMBL5414359 IC50 = 0.49 nM 9.31
CHEMBL5416117 IC50 = 0.63 nM 9.20
CHEMBL5396725 IC50 = 0.7 nM 9.15
CHEMBL5402477 IC50 = 0.71 nM 9.15
CHEMBL5411825 IC50 = 0.83 nM 9.08
CHEMBL5440319 IC50 = 0.88 nM 9.06
CHEMBL5416758 IC50 = 0.9 nM 9.05
CHEMBL5425043 IC50 = 1.5 nM 8.82
CHEMBL5423457 IC50 = 3.29 nM 8.48
CHEMBL5429467 IC50 = 4.82 nM 8.32
CHEMBL5417604 IC50 = 5.4 nM 8.27
CHEMBL5432423 IC50 = 6.14 nM 8.21
CHEMBL5427899 IC50 = 7.32 nM 8.13
CHEMBL4225966 SEL-120 FREE BASE IC50 = 14.11 nM 7.85
CHEMBL5416134 IC50 = 17.1 nM 7.77
CHEMBL5412471 IC50 = 35.7 nM 7.45
CHEMBL5397969 IC50 = 80.0 nM 7.10
CHEMBL5436530 IC50 = 82.9 nM 7.08
CHEMBL5402190 IC50 = 277.0 nM 6.56
CHEMBL5406025 IC50 > 1000.0 nM -