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Assay Detail

CHEMBL5388308

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Positive allosteric modulation at AMPAR in HEK293 cells expressing GluA2Q assessed as glutamate induced calcium influx
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

Glutamate receptor ionotropic AMPA (CHEMBL2096670)
Type PROTEIN COMPLEX GROUP
Organism Homo sapiens

Publication

New insights in the development of positive allosteric modulators of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors belonging to 3,4-dihydro-2H-1,2,4-benzothiadiazine 1,1-dioxides: Introduction of (mono/difluoro)methyl groups at the 2-position of the thiadiazine ring.
Goffin E, Fraikin P, Abboud D, de Tullio P, Beaufour C, Botez I, Hanson J, Danober L, Francotte P, Pirotte B.
Eur J Med Chem (2023) 250 : 115221 -115221
PubMed 36863228 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 17 5.28 6.56

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2441067 1 6.56
CHEMBL5396768 1 6.55
CHEMBL2441068 1 6.10
CHEMBL5400708 1 5.67
CHEMBL5419553 1 5.66
CHEMBL5397532 1 5.06
CHEMBL5403565 1 4.82
CHEMBL5410137 1 4.78
CHEMBL5427188 1 4.50
CHEMBL5416254 1 4.24
CHEMBL4073431 1 4.09
CHEMBL5431136 1 -
CHEMBL5400980 1 -
CHEMBL5408760 1 -
CHEMBL5404910 1 -
CHEMBL5409424 1 -
CHEMBL5397405 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2441067 EC50 = 275.42 nM 6.56
CHEMBL5396768 EC50 = 281.84 nM 6.55
CHEMBL2441068 EC50 = 794.33 nM 6.10
CHEMBL5400708 EC50 = 2137.96 nM 5.67
CHEMBL5419553 EC50 = 2187.76 nM 5.66
CHEMBL5397532 EC50 = 8709.64 nM 5.06
CHEMBL5403565 EC50 = 15135.61 nM 4.82
CHEMBL5410137 EC50 = 16595.87 nM 4.78
CHEMBL5427188 EC50 = 31622.78 nM 4.50
CHEMBL5416254 EC50 = 57543.99 nM 4.24
CHEMBL4073431 EC50 = 81283.05 nM 4.09
CHEMBL5431136 EC50 > 100000.0 nM -
CHEMBL5400980 EC50 > 100000.0 nM -
CHEMBL5408760 EC50 > 100000.0 nM -
CHEMBL5404910 EC50 > 100000.0 nM -
CHEMBL5409424 EC50 > 100000.0 nM -
CHEMBL5397405 EC50 > 100000.0 nM -