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Assay Detail

CHEMBL5389261

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at rat TRPM8 transfected in HEK293 cells assessed as inhibition of icilin induced increase in intracellular Ca2+ concentration preincubated for 5 mins followed by icilin stimulation by Fluo4-AM dye based assay
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Phytochemical Characterization and TRPA1/TRPM8 Modulation Profile of the Cannabigerol-Rich <i>Cannabis sativa</i> L. Chemotype IV.
Gargiulo E, Moriello AS, Benetti E, Pagni L, Arnoldi L, De Petrocellis L, Chianese G, Vitale RM, Taglialatela-Scafati O.
J Nat Prod (2024) 87 : 722 -732
PubMed 38408345 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 12 5.29 6.80

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL497318 CANNABIGEROL 2.0 1 6.80
CHEMBL5421918 1 6.10
CHEMBL463843 CANNABIGEROLIC ACID 1 5.88
CHEMBL4636830 1 5.52
CHEMBL5433171 1 5.40
CHEMBL5410813 1 5.28
CHEMBL5424611 1 5.10
CHEMBL5415604 1 4.98
CHEMBL5437877 1 4.76
CHEMBL5432734 1 4.62
CHEMBL5404545 1 4.53
CHEMBL5404503 1 4.48

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL497318 CANNABIGEROL IC50 = 160.0 nM 6.80
CHEMBL5421918 IC50 = 800.0 nM 6.10
CHEMBL463843 CANNABIGEROLIC ACID IC50 = 1310.0 nM 5.88
CHEMBL4636830 IC50 = 3000.0 nM 5.52
CHEMBL5433171 IC50 = 4000.0 nM 5.40
CHEMBL5410813 IC50 = 5200.0 nM 5.28
CHEMBL5424611 IC50 = 8000.0 nM 5.10
CHEMBL5415604 IC50 = 10400.0 nM 4.98
CHEMBL5437877 IC50 = 17300.0 nM 4.76
CHEMBL5432734 IC50 = 23900.0 nM 4.62
CHEMBL5404545 IC50 = 29500.0 nM 4.53
CHEMBL5404503 IC50 = 33000.0 nM 4.48