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Assay Detail

CHEMBL5442187

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
PDSP Secondary Binding target: OPRK1 - Compounds are tested at 10 uM concentration, plate are incubated at room temperature in the dark for 90 minutes. Reaction are stopped by vacuum filtration onto 0.3% polyethyleneimine soaked filter mats using Filtermate harvester. Scintillation cocktail is then melted onto microwave-dried filters on a hot plate and the radio activity is counted in Microbia counter. Compounds showing a minimum of 50% inhibition at 10 uM concentration are carried forward in this secondary binding assay to determine equilibrium binding affinity.
32
Total Activities
32
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Category PDSP assay
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Kappa-type opioid receptor (CHEMBL237)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

EUbOPEN Chemogenomics Library - PDSP Secondary Binding
EUbOPEN
(2024)
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 32 6.57 9.23

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4750757 1 9.23
CHEMBL1256750 1 8.75
CHEMBL1255661 1 8.67
CHEMBL5076917 1 8.39
CHEMBL25230 1 7.63
CHEMBL1707 LOPERAMIDE HYDROCHLORIDE 4.0 1 7.14
CHEMBL513136 1 7.12
CHEMBL74355 VOLINANSERIN 3.0 1 6.99
CHEMBL1474387 1 6.93
CHEMBL2018875 1 6.71
CHEMBL405355 NIGULDIPINE 2.0 1 6.46
CHEMBL3039520 LASMIDITAN 4.0 1 6.45
CHEMBL4202825 1 6.39
CHEMBL1256844 1 6.35
CHEMBL1200776 CINACALCET HYDROCHLORIDE 4.0 1 6.35
CHEMBL256470 1 6.31
CHEMBL259656 1 6.30
CHEMBL2110363 1 6.15
CHEMBL300555 SPIROXATRINE 2.0 1 6.15
CHEMBL542139 1 6.05
CHEMBL79834 SPIRAMIDE 2.0 1 6.04
CHEMBL3342358 1 6.00
CHEMBL2391908 1 5.96
CHEMBL2181226 1 5.95
CHEMBL103769 1 5.95
CHEMBL2107572 TECALCET HYDROCHLORIDE 2.0 1 5.85
CHEMBL267777 RITANSERIN 2.0 1 5.84
CHEMBL59898 1 5.71
CHEMBL360648 1 5.69
CHEMBL1600943 1 5.67

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4750757 Ki = 0.5913 nM 9.23
CHEMBL1256750 Ki = 1.792 nM 8.75
CHEMBL1255661 Ki = 2.143 nM 8.67
CHEMBL5076917 Ki = 4.034 nM 8.39
CHEMBL25230 Ki = 23.34 nM 7.63
CHEMBL1707 LOPERAMIDE HYDROCHLORIDE Ki = 71.57 nM 7.14
CHEMBL513136 Ki = 76.12 nM 7.12
CHEMBL74355 VOLINANSERIN Ki = 102.71 nM 6.99
CHEMBL1474387 Ki = 118.1 nM 6.93
CHEMBL2018875 Ki = 194.98 nM 6.71
CHEMBL405355 NIGULDIPINE Ki = 346.85 nM 6.46
CHEMBL3039520 LASMIDITAN Ki = 356.57 nM 6.45
CHEMBL4202825 Ki = 406.53 nM 6.39
CHEMBL1256844 Ki = 447.61 nM 6.35
CHEMBL1200776 CINACALCET HYDROCHLORIDE Ki = 444.22 nM 6.35
CHEMBL256470 Ki = 493.97 nM 6.31
CHEMBL259656 Ki = 502.75 nM 6.30
CHEMBL2110363 Ki = 703.8 nM 6.15
CHEMBL300555 SPIROXATRINE Ki = 711.3 nM 6.15
CHEMBL542139 Ki = 898.05 nM 6.05
CHEMBL79834 SPIRAMIDE Ki = 913.9 nM 6.04
CHEMBL3342358 Ki = 997.55 nM 6.00
CHEMBL2391908 Ki = 1110.2 nM 5.96
CHEMBL2181226 Ki = 1133.31 nM 5.95
CHEMBL103769 Ki = 1113.27 nM 5.95
CHEMBL2107572 TECALCET HYDROCHLORIDE Ki = 1403.62 nM 5.85
CHEMBL267777 RITANSERIN Ki = 1445.44 nM 5.84
CHEMBL59898 Ki = 1941.19 nM 5.71
CHEMBL360648 Ki = 2048.33 nM 5.69
CHEMBL1600943 Ki = 2122.51 nM 5.67
CHEMBL502182 ELAGOLIX SODIUM Ki = 2383.41 nM 5.62
CHEMBL83894 FANANSERIN Ki = 2874.09 nM 5.54