Assay Detail
Binding
CHEMBL5442199
Review assay metadata, readout intent, target linkage, and publication context from the same page.
PDSP Secondary Binding target: HRH3 - Compounds are tested at 10 uM concentration, plate are incubated at room temperature in the dark for 90 minutes. Reaction are stopped by vacuum filtration onto 0.3% polyethyleneimine soaked filter mats using Filtermate harvester. Scintillation cocktail is then melted onto microwave-dried filters on a hot plate and the radio activity is counted in Microbia counter. Compounds showing a minimum of 50% inhibition at 10 uM concentration are carried forward in this secondary binding assay to determine equilibrium binding affinity.
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Category | PDSP assay |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
EUbOPEN Chemogenomics Library - PDSP Secondary Binding
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 12 | 7.27 | 9.20 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1490875 | — | — | 1 | 9.20 |
| CHEMBL5077041 | — | — | 1 | 9.20 |
| CHEMBL1434496 | — | — | 1 | 8.58 |
| CHEMBL18442 | PLERIXAFOR | 4.0 | 1 | 8.56 |
| CHEMBL4164059 | PITOLISANT HYDROCHLORIDE | 4.0 | 1 | 8.36 |
| CHEMBL5076314 | — | — | 1 | 7.08 |
| CHEMBL103769 | — | — | 1 | 6.98 |
| CHEMBL2018875 | — | — | 1 | 6.19 |
| CHEMBL5089005 | — | — | 1 | 6.15 |
| CHEMBL5075931 | — | — | 1 | 5.70 |
| CHEMBL405355 | NIGULDIPINE | 2.0 | 1 | 5.67 |
| CHEMBL2105760 | BREXPIPRAZOLE | 4.0 | 1 | 5.58 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1490875 | — | Ki | = | 0.6325 | nM | 9.20 |
| CHEMBL5077041 | — | Ki | = | 0.6296 | nM | 9.20 |
| CHEMBL1434496 | — | Ki | = | 2.627 | nM | 8.58 |
| CHEMBL18442 | PLERIXAFOR | Ki | = | 2.744 | nM | 8.56 |
| CHEMBL4164059 | PITOLISANT HYDROCHLORIDE | Ki | = | 4.361 | nM | 8.36 |
| CHEMBL5076314 | — | Ki | = | 82.42 | nM | 7.08 |
| CHEMBL103769 | — | Ki | = | 104.45 | nM | 6.98 |
| CHEMBL2018875 | — | Ki | = | 645.06 | nM | 6.19 |
| CHEMBL5089005 | — | Ki | = | 709.17 | nM | 6.15 |
| CHEMBL5075931 | — | Ki | = | 1975.83 | nM | 5.70 |
| CHEMBL405355 | NIGULDIPINE | Ki | = | 2135.5 | nM | 5.67 |
| CHEMBL2105760 | BREXPIPRAZOLE | Ki | = | 2607.35 | nM | 5.58 |