Assay Detail
Binding
CHEMBL5442203
Review assay metadata, readout intent, target linkage, and publication context from the same page.
PDSP Secondary Binding target: ADRA1A - Compounds are tested at 10 uM concentration, plate are incubated at room temperature in the dark for 90 minutes. Reaction are stopped by vacuum filtration onto 0.3% polyethyleneimine soaked filter mats using Filtermate harvester. Scintillation cocktail is then melted onto microwave-dried filters on a hot plate and the radio activity is counted in Microbia counter. Compounds showing a minimum of 50% inhibition at 10 uM concentration are carried forward in this secondary binding assay to determine equilibrium binding affinity.
27
Total Activities
27
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Category | PDSP assay |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
EUbOPEN Chemogenomics Library - PDSP Secondary Binding
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 27 | 6.64 | 8.79 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1558 | PRAZOSIN HYDROCHLORIDE | 4.0 | 1 | 8.79 |
| CHEMBL405355 | NIGULDIPINE | 2.0 | 1 | 8.17 |
| CHEMBL540612 | — | — | 1 | 7.98 |
| CHEMBL2105760 | BREXPIPRAZOLE | 4.0 | 1 | 7.53 |
| CHEMBL24778 | SILODOSIN | 4.0 | 1 | 7.52 |
| CHEMBL1200517 | DIHYDROERGOTAMINE MESYLATE | 4.0 | 1 | 7.48 |
| CHEMBL1200492 | NEFAZODONE HYDROCHLORIDE | 4.0 | 1 | 6.86 |
| CHEMBL267777 | RITANSERIN | 2.0 | 1 | 6.76 |
| CHEMBL83894 | FANANSERIN | 2.0 | 1 | 6.71 |
| CHEMBL2024517 | CARIPRAZINE HYDROCHLORIDE | 4.0 | 1 | 6.62 |
| CHEMBL1615374 | VILAZODONE HYDROCHLORIDE | 4.0 | 1 | 6.56 |
| CHEMBL1095777 | INDACATEROL | 4.0 | 1 | 6.45 |
| CHEMBL79834 | SPIRAMIDE | 2.0 | 1 | 6.39 |
| CHEMBL589390 | LATREPIRDINE | 3.0 | 1 | 6.38 |
| CHEMBL300555 | SPIROXATRINE | 2.0 | 1 | 6.35 |
| CHEMBL1200809 | AZELASTINE HYDROCHLORIDE | 4.0 | 1 | 6.30 |
| CHEMBL103769 | — | — | 1 | 6.29 |
| CHEMBL1263 | SALMETEROL | 4.0 | 1 | 6.26 |
| CHEMBL1705 | CLONIDINE HYDROCHLORIDE | 4.0 | 1 | 6.24 |
| CHEMBL445102 | — | — | 1 | 6.20 |
| CHEMBL1257131 | RAUWOLSCINE HYDROCHLORIDE | — | 1 | 6.15 |
| CHEMBL2106195 | DEXMEDETOMIDINE HYDROCHLORIDE | 4.0 | 1 | 6.13 |
| CHEMBL5075931 | — | — | 1 | 5.96 |
| CHEMBL1489369 | — | — | 1 | 5.83 |
| CHEMBL5076314 | — | — | 1 | 5.80 |
| CHEMBL1516474 | TEGASEROD MALEATE | 4.0 | 1 | 5.79 |
| CHEMBL1256998 | — | — | 1 | 5.73 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1558 | PRAZOSIN HYDROCHLORIDE | Ki | = | 1.626 | nM | 8.79 |
| CHEMBL405355 | NIGULDIPINE | Ki | = | 6.752 | nM | 8.17 |
| CHEMBL540612 | — | Ki | = | 10.48 | nM | 7.98 |
| CHEMBL2105760 | BREXPIPRAZOLE | Ki | = | 29.27 | nM | 7.53 |
| CHEMBL24778 | SILODOSIN | Ki | = | 30.28 | nM | 7.52 |
| CHEMBL1200517 | DIHYDROERGOTAMINE MESYLATE | Ki | = | 32.9 | nM | 7.48 |
| CHEMBL1200492 | NEFAZODONE HYDROCHLORIDE | Ki | = | 137.29 | nM | 6.86 |
| CHEMBL267777 | RITANSERIN | Ki | = | 172.44 | nM | 6.76 |
| CHEMBL83894 | FANANSERIN | Ki | = | 194.94 | nM | 6.71 |
| CHEMBL2024517 | CARIPRAZINE HYDROCHLORIDE | Ki | = | 242.57 | nM | 6.62 |
| CHEMBL1615374 | VILAZODONE HYDROCHLORIDE | Ki | = | 278.07 | nM | 6.56 |
| CHEMBL1095777 | INDACATEROL | Ki | = | 355.59 | nM | 6.45 |
| CHEMBL79834 | SPIRAMIDE | Ki | = | 405.13 | nM | 6.39 |
| CHEMBL589390 | LATREPIRDINE | Ki | = | 414.33 | nM | 6.38 |
| CHEMBL300555 | SPIROXATRINE | Ki | = | 445.96 | nM | 6.35 |
| CHEMBL1200809 | AZELASTINE HYDROCHLORIDE | Ki | = | 495.79 | nM | 6.30 |
| CHEMBL103769 | — | Ki | = | 512.51 | nM | 6.29 |
| CHEMBL1263 | SALMETEROL | Ki | = | 548.66 | nM | 6.26 |
| CHEMBL1705 | CLONIDINE HYDROCHLORIDE | Ki | = | 575.64 | nM | 6.24 |
| CHEMBL445102 | — | Ki | = | 635.11 | nM | 6.20 |
| CHEMBL1257131 | RAUWOLSCINE HYDROCHLORIDE | Ki | = | 705.75 | nM | 6.15 |
| CHEMBL2106195 | DEXMEDETOMIDINE HYDROCHLORIDE | Ki | = | 749.03 | nM | 6.13 |
| CHEMBL5075931 | — | Ki | = | 1096.48 | nM | 5.96 |
| CHEMBL1489369 | — | Ki | = | 1467.57 | nM | 5.83 |
| CHEMBL5076314 | — | Ki | = | 1584.53 | nM | 5.80 |
| CHEMBL1516474 | TEGASEROD MALEATE | Ki | = | 1632.3 | nM | 5.79 |
| CHEMBL1256998 | — | Ki | = | 1849.05 | nM | 5.73 |