Assay Detail
Functional
CHEMBL5445900
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Affinity Phenotypic Cellular interaction: (Cytotoxicity sulforhodamine B assay (growth inhibitory activity in human colorectal cancer cell line COLO205, B-Raf mutation)) EUB0000124b MAP2K1
1
Total Activities
1
Compounds Tested
1
Activity Types
3
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Homo sapiens |
| Cell Type | COLO 205 |
| Category | Affinity phenotypic cellular assay |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Dual specificity mitogen-activated protein kinase kinase 1 (CHEMBL3587) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Affinity Phenotypic Cellular Literature for EUbOPEN Chemogenomic Library
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 1 | 9.28 | 9.28 |
Assay Parameters
| Parameter | Type | Value | Units | Text |
|---|---|---|---|---|
| Mode of action | Mode of action | - | — | Inhibitor |
| Protein family | Protein family | - | — | Protein kinase |
| Recommended concentration | Recommended concentration | 100.0 | nM | — |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL2103875 | TRAMETINIB | 4.0 | 1 | 9.28 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL2103875 | TRAMETINIB | IC50 | = | 0.52 | nM | 9.28 |