Assay Detail
Binding
CHEMBL5464409
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Inhibition of Oxytocin at 1 µM in the Eurofins-Panlabs radioligand binding assay (Eurofins_assay_260900)
8
Total Activities
2
Compounds Tested
3
Activity Types
10
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Profiling data from Eurofins-Panlabs
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Inhibition | 6 | - | - |
| IC50 | 1 | 7.81 | 7.81 |
| Ki | 1 | 8.30 | 8.30 |
Assay Parameters
| Parameter | Type | Value | Units | Text |
|---|---|---|---|---|
| Detection method | Detection method | - | — | Radioligand Binding |
| Incubation temperature | Incubation temperature | - | — | 25°C |
| Incubation time | Incubation time | - | — | 2 h |
| Ligand | Ligand | - | — | [³H] Oxytocin |
| Ligand concentration | Ligand concentration | 0.8 | nM | — |
| Ligand Kd | Ligand Kd | 0.39 | nM | — |
| Non-specific ligand with concentration | Non-specific ligand with concentration | - | — | 1 µM Oxytocin |
| Reference compound | Reference compound | - | — | Oxytocin |
| reference compound IC50 | reference compound IC50 | 950.0 | nM | — |
| Source of protein | Source of protein | - | — | Human recombinant Chem-1 (rat hematopoetic) cells |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4594444 | PECAVAPTAN | 2.0 | 7 | 8.30 |
| CHEMBL5465173 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4594444 | PECAVAPTAN | Ki | = | 5.06 | nM | 8.30 |
| CHEMBL4594444 | PECAVAPTAN | IC50 | = | 15.5 | nM | 7.81 |
| CHEMBL4594444 | PECAVAPTAN | Inhibition | = | 95.0 | % | - |
| CHEMBL4594444 | PECAVAPTAN | Inhibition | = | 84.0 | % | - |
| CHEMBL4594444 | PECAVAPTAN | Inhibition | = | 41.0 | % | - |
| CHEMBL4594444 | PECAVAPTAN | Inhibition | = | 6.0 | % | - |
| CHEMBL4594444 | PECAVAPTAN | Inhibition | = | 3.0 | % | - |
| CHEMBL5465173 | — | Inhibition | = | 12.0 | % | - |