Assay Detail
Binding
CHEMBL5502632
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Binding affinity to recombinant His-tagged STAT3 (127 to 722 residues) (unknown origin) expressed in Escherichia coli BL-21 Star (De3) pLysS by bio-layer interferometry assay
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Signal transducer and activator of transcription 3 (CHEMBL4026) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Exploration of novel isoxazole-fused quinone derivatives as anti-colorectal cancer agents through inhibiting STAT3 and elevating ROS level.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Kd | 10 | 4.91 | 5.36 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5558267 | — | — | 1 | 5.36 |
| CHEMBL5557203 | — | — | 1 | 5.35 |
| CHEMBL5555159 | — | — | 1 | 5.30 |
| CHEMBL5517930 | — | — | 1 | 5.25 |
| CHEMBL64130 | NAPABUCASIN | 3.0 | 1 | 4.89 |
| CHEMBL5550059 | — | — | 1 | 4.54 |
| CHEMBL5556837 | — | — | 1 | 4.37 |
| CHEMBL5518134 | — | — | 1 | 4.19 |
| CHEMBL5532068 | — | — | 1 | - |
| CHEMBL5505783 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5558267 | — | Kd | = | 4400.0 | nM | 5.36 |
| CHEMBL5557203 | — | Kd | = | 4500.0 | nM | 5.35 |
| CHEMBL5555159 | — | Kd | = | 5000.0 | nM | 5.30 |
| CHEMBL5517930 | — | Kd | = | 5600.0 | nM | 5.25 |
| CHEMBL64130 | NAPABUCASIN | Kd | = | 13000.0 | nM | 4.89 |
| CHEMBL5550059 | — | Kd | = | 29000.0 | nM | 4.54 |
| CHEMBL5556837 | — | Kd | = | 43000.0 | nM | 4.37 |
| CHEMBL5518134 | — | Kd | = | 65000.0 | nM | 4.19 |
| CHEMBL5532068 | — | Kd | - | — | - | |
| CHEMBL5505783 | — | Kd | - | — | - |