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Assay Detail

CHEMBL5502958

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of BTK (unknown origin) using fluorescein labeled polyGAT peptide substrate incubated for 30 mins in presence of ATP by time-resolved fluorescence assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein kinase BTK (CHEMBL5251)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery and Preclinical Characterization of BIIB129, a Covalent, Selective, and Brain-Penetrant BTK Inhibitor for the Treatment of Multiple Sclerosis.
Himmelbauer MK, Bajrami B, Basile R, Capacci A, Chen T, Choi CK, Gilfillan R, Gonzalez-Lopez de Turiso F, Gu C, Hoemberger M, Johnson DS, Jones JH, Kadakia E, Kirkland M, Lin EY, Liu Y, Ma B, Magee T, Mantena S, Marx IE, Metrick CM, Mingueneau M, Murugan P, Muste CA, Nadella P, Nevalainen M, Parker Harp CR, Pattaropong V, Pietrasiewicz A, Prince RJ, Purgett TJ, Santoro JC, Schulz J, Sciabola S, Tang H, Vandeveer HG, Wang T, Yousaf Z, Helal CJ, Hopkins BT.
J Med Chem (2024) 67 : 8122 -8140
PubMed 38712838 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 8.33 9.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5558638 1 9.30
CHEMBL5549935 1 8.47
CHEMBL5505806 1 8.34
CHEMBL5555158 1 7.20

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5558638 IC50 = 0.5 nM 9.30
CHEMBL5549935 IC50 = 3.4 nM 8.47
CHEMBL5505806 IC50 = 4.6 nM 8.34
CHEMBL5555158 IC50 = 63.0 nM 7.20