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Assay Detail

CHEMBL5505507

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of C-terminal His-tagged SARS-CoV-2 PLpro BetaCoV/Wuhan/WIV04/2019 expressed in Escherichia coli BL21(DE3) using ISG-AMC substrate incubated for 3 hrs by FRET analysis
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Severe acute respiratory syndrome coronavirus 2
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Replicase polyprotein 1ab (CHEMBL4523582)
Type SINGLE PROTEIN
Organism Severe acute respiratory syndrome coronavirus 2

Publication

New Strategies for Responding to SARS-CoV-2: The Present and Future of Dual-Target Drugs.
Zhang W, Xiao L, Li D, Hu Y, Yu W.
J Med Chem (2024) 67 : 11522 -11542
PubMed 38967785 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 7.52 7.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4852855 1 7.92
CHEMBL2315036 1 7.70
CHEMBL5557204 1 7.22
CHEMBL5542652 1 7.22

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4852855 IC50 = 12.0 nM 7.92
CHEMBL2315036 IC50 = 20.0 nM 7.70
CHEMBL5557204 IC50 = 60.0 nM 7.22
CHEMBL5542652 IC50 = 60.0 nM 7.22