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Assay Detail

CHEMBL5507325

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human TEV fused AKR1C3 expressed in Escherichia coli BL21(DE) codon plus RP cells assessed as reduction in substrate oxidation using S-tetralol as substrate in presence of NADP+
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Aldo-keto reductase family 1 member C3 (CHEMBL4681)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-guided optimization of 3-hydroxybenzoisoxazole derivatives as inhibitors of Aldo-keto reductase 1C3 (AKR1C3) to target prostate cancer.
Pippione AC, Kovachka S, Vigato C, Bertarini L, Mannella I, Sainas S, Rolando B, Denasio E, Piercy-Mycock H, Romalho L, Salladini E, Adinolfi S, Zonari D, Peraldo-Neia C, Chiorino G, Passoni A, Mirza OA, Frydenvang K, Pors K, Lolli ML, Spyrakis F, Oliaro-Bosso S, Boschi D.
Eur J Med Chem (2024) 268 : 116193 -116193
PubMed 38364714 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 17 6.76 7.36

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4513510 ASP-9521 1.0 1 7.36
CHEMBL5532410 1 7.34
CHEMBL5561463 1 7.22
CHEMBL5555217 1 7.17
CHEMBL5557454 1 7.00
CHEMBL5561484 1 6.96
CHEMBL5558400 1 6.92
CHEMBL5566714 1 6.80
CHEMBL5562651 1 6.72
CHEMBL5517678 1 6.72
CHEMBL4238437 1 6.58
CHEMBL5559780 1 6.57
CHEMBL5559881 1 6.46
CHEMBL5560443 1 6.38
CHEMBL5556734 1 6.29
CHEMBL5557319 1 6.29
CHEMBL5557212 1 6.20

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4513510 ASP-9521 IC50 = 44.0 nM 7.36
CHEMBL5532410 IC50 = 46.0 nM 7.34
CHEMBL5561463 IC50 = 60.0 nM 7.22
CHEMBL5555217 IC50 = 68.0 nM 7.17
CHEMBL5557454 IC50 = 100.0 nM 7.00
CHEMBL5561484 IC50 = 110.0 nM 6.96
CHEMBL5558400 IC50 = 120.0 nM 6.92
CHEMBL5566714 IC50 = 160.0 nM 6.80
CHEMBL5562651 IC50 = 190.0 nM 6.72
CHEMBL5517678 IC50 = 190.0 nM 6.72
CHEMBL4238437 IC50 = 260.0 nM 6.58
CHEMBL5559780 IC50 = 270.0 nM 6.57
CHEMBL5559881 IC50 = 350.0 nM 6.46
CHEMBL5560443 IC50 = 420.0 nM 6.38
CHEMBL5556734 IC50 = 510.0 nM 6.29
CHEMBL5557319 IC50 = 510.0 nM 6.29
CHEMBL5557212 IC50 = 630.0 nM 6.20