Assay Detail
Binding
CHEMBL5508580
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of amino-terminal His-SUMO tagged SARS-CoV-2 3CLpro using HilyteFluor 488-Glu-Ser-Ala-Thr-Leu-Gln-Ser-Gly-Leu-Arg-Lys-Ala-Lys-QXL 520 as FRET peptide substrate preincubated for 90 mins followed by substrate addition and measured over 30 mins by continuous FRET assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Severe acute respiratory syndrome coronavirus 2 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Replicase polyprotein 1ab (CHEMBL4523582) ↗| Type | SINGLE PROTEIN |
| Organism | Severe acute respiratory syndrome coronavirus 2 |
Publication
Synthesis and biological evaluation of novel peptidomimetic inhibitors of the coronavirus 3C-like protease.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 2 | 6.63 | 6.78 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5532059 | — | — | 1 | 6.78 |
| CHEMBL4802135 | NIRMATRELVIR | 4.0 | 1 | 6.48 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5532059 | — | IC50 | = | 165.7 | nM | 6.78 |
| CHEMBL4802135 | NIRMATRELVIR | IC50 | = | 332.7 | nM | 6.48 |