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Assay Detail

CHEMBL5511547

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant wildtype human N-terminal GST-tagged EGFR cytoplasmic domain (669 to 1210 residues) expressed in Sf21 insect cells using Poly(Glu,Tyr) peptide as substrate preincubated for 15 mins followed by substrate addition and measured after 50 mins in presence of ATP by ADP-Glo reagent based assay
32
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf21
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Epidermal growth factor receptor (CHEMBL203)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery and Optimization of Potent, Efficacious and Selective Inhibitors Targeting EGFR Exon20 Insertion Mutations.
Thomson C, Barton P, Braybrooke E, Colclough N, Dong Z, Evans L, Floc'h N, Guérot C, Hargreaves D, Khurana P, Li S, Li X, Lister A, McCoull W, McWilliams L, Orme JP, Packer MJ, Swaih AM, Ward RA, Winlow P, Ye Y.
J Med Chem (2024) 67 : 8988 -9027
PubMed 38770784 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 32 7.84 9.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5567575 2 9.00
CHEMBL5561612 2 9.00
CHEMBL4650319 MOBOCERTINIB 4.0 2 9.00
CHEMBL5558799 2 8.70
CHEMBL5559367 2 8.70
CHEMBL5542205 2 8.22
CHEMBL5567954 2 8.00
CHEMBL5556548 2 7.68
CHEMBL5562889 2 7.46
CHEMBL5563422 2 7.44
CHEMBL5567060 2 7.23
CHEMBL5561729 2 7.02
CHEMBL5566816 2 6.43
CHEMBL5517919 2 5.82
CHEMBL5562445 2 -
CHEMBL4650281 CLN-081 3.0 2 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5567575 IC50 = 1.0 nM 9.00
CHEMBL5561612 IC50 = 1.0 nM 9.00
CHEMBL4650319 MOBOCERTINIB IC50 = 1.0 nM 9.00
CHEMBL5558799 IC50 = 2.0 nM 8.70
CHEMBL5559367 IC50 = 2.0 nM 8.70
CHEMBL5542205 IC50 = 6.0 nM 8.22
CHEMBL5567954 IC50 = 10.0 nM 8.00
CHEMBL5556548 IC50 = 21.0 nM 7.68
CHEMBL5562889 IC50 = 35.0 nM 7.46
CHEMBL5563422 IC50 = 36.0 nM 7.44
CHEMBL5567060 IC50 = 59.0 nM 7.23
CHEMBL5561729 IC50 = 95.0 nM 7.02
CHEMBL5566816 IC50 = 370.0 nM 6.43
CHEMBL5517919 IC50 = 1500.0 nM 5.82
CHEMBL4650281 CLN-081 IC50 < 3.0 nM -
CHEMBL5562445 IC50 > 794328234.72 nM -
CHEMBL5567060 IC50 > 794328234.72 nM -
CHEMBL5561729 IC50 > 794328234.72 nM -
CHEMBL5566816 IC50 > 794328234.72 nM -
CHEMBL5562889 IC50 > 676082975.39 nM -
CHEMBL5558799 IC50 > 676082975.39 nM -
CHEMBL5567575 IC50 > 676082975.39 nM -
CHEMBL5556548 IC50 > 676082975.39 nM -
CHEMBL5567954 IC50 > 676082975.39 nM -
CHEMBL5561612 IC50 > 676082975.39 nM -
CHEMBL5559367 IC50 > 676082975.39 nM -
CHEMBL5563422 IC50 > 676082975.39 nM -
CHEMBL5517919 IC50 > 933254300.8 nM -
CHEMBL5542205 IC50 > 933254300.8 nM -
CHEMBL4650319 MOBOCERTINIB IC50 > 933254300.8 nM -
CHEMBL4650281 CLN-081 IC50 > 933254300.8 nM -
CHEMBL5562445 IC50 > 7400.0 nM -