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Assay Detail

CHEMBL5513574

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of NHE1 in human Platelet assessed as change in intracellular pH by pH-sensitive BCECF-AM fluorescence probe method
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Platelet
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sodium/hydrogen exchanger 1 (CHEMBL2781)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

pH regulators and their inhibitors in tumor microenvironment.
Liao S, Wu G, Xie Z, Lei X, Yang X, Huang S, Deng X, Wang Z, Tang G.
Eur J Med Chem (2024) 267 : 116170 -116170
PubMed 38308950 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 6.94 7.23

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL355862 ZONIPORIDE 2.0 1 7.23
CHEMBL5562354 1 7.07
CHEMBL1256180 5-(N-METHYL-N-ISOBUTYL)AMILORIDE 1 6.76
CHEMBL3526475 1 6.70

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL355862 ZONIPORIDE IC50 = 59.0 nM 7.23
CHEMBL5562354 IC50 = 85.0 nM 7.07
CHEMBL1256180 5-(N-METHYL-N-ISOBUTYL)AMILORIDE IC50 = 174.0 nM 6.76
CHEMBL3526475 IC50 = 200.0 nM 6.70