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Assay Detail

CHEMBL5520159

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of full length human USP7 (1 to 1102 residues) expressed in Escherichia coli BL21 (DE3) using Ubiquitin-AMC as substrate pre-incubated for 30 mins followed by substrate addition measured for 60 mins by fluorescence based analysis
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Ubiquitin carboxyl-terminal hydrolase 7 (CHEMBL2157850)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Recent advances in the development of deubiquitinases inhibitors as antitumor agents.
Zheng LL, Wang LT, Pang YW, Sun LP, Shi L.
Eur J Med Chem (2024) 266 : 116161 -116161
PubMed 38262120 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 6.05 6.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2159505 1 6.40
CHEMBL2159500 1 5.70

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2159505 IC50 = 400.0 nM 6.40
CHEMBL2159500 IC50 = 2000.0 nM 5.70